![Western Blot: Cytoglobin Antibody (1A1) [H00114757-M02] - Analysis of CYGB expression in transfected 293T cell line by CYGB monoclonal antibody (M02), clone 1A1.Lane 1: CYGB transfected lysate(21 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Cytoglobin-Antibody-1A1-Western-Blot-H00114757-M02-img0009.jpg "Western Blot: Cytoglobin Antibody (1A1) [H00114757-M02] - Analysis of CYGB expression in transfected 293T cell line by CYGB monoclonal antibody (M02), clone 1A1.Lane 1: CYGB transfected lysate(21 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 1A1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | CYGB (AAH29798, 1 a.a. ~ 190 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEKVPGEMEIERRERSEELSEAERKAVQAMWARLYASCEDVGVAILVRFFVNFPSAKQYFSQFKHMEDPLEMERSPQLRKHACRVMGALNTVVENLHDPDKVSSVLALVGKAHALKHKVEPVYFKILSGVILEVVAEEFASDFPPETQRAWAKLRGLIYSHVTAAYKEVGWVQQVPNATTPPATLPSSGP |
| Specificity | CYGB - cytoglobin |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CYGB |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against transfected lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), immunofluorescence, and ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
![Myoglobin Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Myoglobin Antibody [Unconjugated]")
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Cytoglobin Antibody (1A1) [H00114757-M02] - Analysis of monoclonal antibody to CYGB on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Cytoglobin-Antibody-1A1-Immunocytochemistry-Immunofluorescence-H00114757-M02-img0005.jpg "Immunocytochemistry/Immunofluorescence: Cytoglobin Antibody (1A1) [H00114757-M02] - Analysis of monoclonal antibody to CYGB on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: Cytoglobin Antibody (1A1) [H00114757-M02] - Analysis of monoclonal antibody to CYGB on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Cytoglobin-Antibody-1A1-Immunohistochemistry-Paraffin-H00114757-M02-img0007.jpg "Immunohistochemistry-Paraffin: Cytoglobin Antibody (1A1) [H00114757-M02] - Analysis of monoclonal antibody to CYGB on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.")
![ELISA: Cytoglobin Antibody (1A1) [H00114757-M02] - Detection limit for recombinant GST tagged CYGB is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cytoglobin-Antibody-1A1-ELISA-H00114757-M02-img0010.jpg "ELISA: Cytoglobin Antibody (1A1) [H00114757-M02] - Detection limit for recombinant GST tagged CYGB is approximately 1ng/ml as a capture antibody.")
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![N/A Erythropoietin/EPO [HRP]](https://images.novusbio.com/images2/Erythropoietin_MEP00_ELISA_421.jpg)
![Bioactivity TGF-beta 1 [Unconjugated]](https://images.novusbio.com/images/protein/7754-bhcf_recombinant-human-tgf-beta-1-human-cell-expressed-cf-bioactivity-1811202013946.jpg)
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")