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Recombinant Human CTLA-4 Fc Chimera His-tag Protein, CF

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Recombinant Human CTLA-4 Fc Chimera (Catalog # 325-CT/CF) inhibits IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.1-0.4 μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1 Fc Chimera (Catalog # 140-B1) in the presence of PHA.
Recombinant Human CTLA-4 Fc Chimera (Catalog # 325-CT/CF) inhibits IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.1-0.4 μg/mL when stimulated with 1 μg/mL ...read more
1 μg/lane of Recombinant Human CTLA-4 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 51 kDa and 94 kDa, respectively ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
325-CT-01M/CF
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Recombinant Human CTLA-4 Fc Chimera His-tag Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. Linsley, P.S. et al. (1991) J. Exp. Med. 174:561. The ED50 for this effect is 0.1-0.4 µg/mL when stimulated with 1 µg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog #
140-B1) in the presence of PHA.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CTLA-4 protein
Human CTLA-4 (Ala37-Phe162)
Accession # Q6GR94		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala37
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
CTLA4
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
40 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
325-CT/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CTLA-4 Fc Chimera His-tag Protein, CF

  • CD
  • CD152 antigen
  • CD152
  • CD152IDDM12
  • CD28
  • celiac disease 3
  • CELIAC3
  • CTLA4
  • CTLA-4
  • cytotoxic T-lymphocyte antigen 4
  • cytotoxic T-lymphocyte protein 4
  • Cytotoxic T-lymphocyte-associated antigen 4
  • cytotoxic T-lymphocyte-associated protein 4
  • cytotoxic T-lymphocyte-associated serine esterase-4
  • GRD4
  • GSE
  • ICOS
  • ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Background

CTLA-4 (cytotoxic T-lymphocyte-4, designated CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily (1, 2). Human or mouse CTLA-4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence, a 126 aa extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane (TM) sequence, and a 41 aa cytoplasmic sequence. It is found as a covalent homodimer of 41‑43 kDa (2). Within the ECD, human CTLA-4 shares 68%, 71% and 83‑86% aa sequence identity with mouse, rat and porcine/bovine/rabbit/feline/canine CTLA-4, respectively. A 174 aa form that lacks TM and cytoplasmic sequences (sCTLA-4) is possibly secreted (3‑5). Isoforms of 56‑79 aa that mainly contain parts of the cytoplasmic domain are reported. In mouse, an isoform lacking the Ig-like domain has ligand-independent inhibitory activity and is termed liCTLA-4 (6). CD28, which is structurally related to CTLA-4, is constitutively expressed on naïve T cells and promotes T cell activation when engaged by B7-2 on antigen-presenting cells (APC) within the immunological synapse (IS) (1, 7, 8). In contrast, CTLA-4 is recruited from intracellular vesicles to the IS beginning 1, 2 days after T cell activation (2, 7, 8). It forms a linear lattice with B7‑1 on APC, inducing negative regulatory signals and ending T cell activation (9). Abatacept, a therapeutic human CTLA-4-Ig fusion protein (trade name Orencia), competes with CD28 for B7-1 and B7-2 binding and has been used to antagonize T cell activation in autoimmune conditions and to enhance transplant survival (10). Mice deleted for CTLA-4 show no abnormalities until after birth, but then develop lethal autoimmune reactions due to continued T cell activation and poor control by regulatory T cells, which constitutively express CTLA-4 in wild-type mice and humans (11‑13).

  1. Harper, K. et al. (1991) J. Immunol. 147:1037.
  2. Teft, W.A. et al. (2006) Annu. Rev. Immunol. 24:65.
  3. Magistrelli, G. et al. (1999) Eur. J. Immunol. 29:3596.
  4. Tector, M. et al. (2009) BMC Immunol. 10:51.
  5. Oaks, M.K. and K.M. Hallett (2000) J. Immunol. 164:5015.
  6. Vijayakrishnan, L. et al. (2004) Immunity 20:563.
  7. Pentcheva-Hoang, T. et al. (2004) Immunity 21:401.
  8. Jansson, A. et al. (2005) J. Immunol 175:1575.
  9. Darlington, P.J. et al. (2005) J. Immunol. 175:996.
  10. Platt, A.M. et al. (2010) J. Immunol. 185:1558.
  11. Wing, K. et al. (2008) Science 322:271.
  12. Friedline, R.H. et al. (2009) J. Exp. Med. 206:421.
  13. Jain, N. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1524.

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Recombinant Human
CTLA-4 Fc Chimera His ...
325-CT/CF
Recombinant Human CTLA-4 Fc Chimera His-tag Protein, CF
Species: Hu
Applications: Bioactivity

Publications for CTLA-4 (325-CT/CF)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Bioassay, ELISA (Standard), Flow Cytometry.


Filter By Application
Bioassay
(5)
ELISA (Standard)
(1)
Flow Cytometry
(1)
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Human
(6)
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Showing Publications 1 - 7 of 7.
Publications using 325-CT/CF Applications Species
BD Hock, SA MacPherson, JL McKenzie Idelalisib and caffeine reduce suppression of T cell responses mediated by activated chronic lymphocytic leukemia cells PLoS ONE, 2017-03-03;12(3):e0172858. 2017-03-03 [PMID: 28257435] (Bioassay, Human) Bioassay Human
Kowalczyk A, D'Souza C, Zhang L Cell-extrinsic CTLA4-mediated regulation of dendritic cell maturation depends on STAT3. Eur J Immunol, 2014-01-22;44(4):1143-55. 2014-01-22 [PMID: 24338929] (Bioassay, Human) Bioassay Human
Steidl C, Shah SP, Woolcock BW MHC class II transactivator CIITA is a recurrent gene fusion partner in lymphoid cancers. Nature, 2011-03-02;471(7338):377-81. 2011-03-02 [PMID: 21368758] (Bioassay, Human) Bioassay Human
Maniar A, Zhang X, Lin W, Gastman BR, Pauza CD, Strome SE, Chapoval AI Human gammadelta T lymphocytes induce robust NK cell-mediated antitumor cytotoxicity through CD137 engagement. Blood, 2010-06-02;116(10):1726-33. 2010-06-02 [PMID: 20519625] (Bioassay, Human) Bioassay Human
Shan M, Cheng HF, Song LZ Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema. Sci Transl Med, 2009-10-28;1(4):4ra10. 2009-10-28 [PMID: 20368170] (Bioassay, Human) Bioassay Human
Sakthivel P, Shively V, Kakoulidou M The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm. J. Intern. Med., 2007-04-01;261(4):399-407. 2007-04-01 [PMID: 17391115] (ELISA (Standard)) ELISA (Standard)
Lazetic S, Leong SR, Chang JC, Ong R, Dawes G, Punnonen J Chimeric co-stimulatory molecules that selectively act through CD28 or CTLA-4 on human T cells. J. Biol. Chem., 2002-08-06;277(41):38660-8. 2002-08-06 [PMID: 12167647] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol CTLA4
Uniprot