Recombinant Human CD84/SLAMF5 Protein Summary
Details of Functionality |
Measured in a cell proliferation assay using PHA stimulated human T cells in the presence of anti-CD3. Tangye, S.G. et al. (2003) J. Immunol. 171:2485. The ED50 for this effect is 1-4 μg/mL in the presence of anti-CD3 immobilized at least at 20 ng/mL. |
Source |
Mouse myeloma cell line, NS0-derived human CD84/SLAMF5 protein Lys22-Arg220, with a C-terminal 10-His tag |
Accession # |
|
N-terminal Sequence |
Lys22 |
Protein/Peptide Type |
Recombinant Proteins |
Gene |
CD84 |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
23.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
40-43 kDa, reducing conditions |
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions |
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human CD84/SLAMF5 Protein
Background
CD84, also known as Ly-9B and SLAMF5, is a type I transmembrane protein in the SLAM subgroup of the CD2 family. SLAM family proteins regulate multiple aspects of immune system function (1). Mature human CD84 consists of a 204 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 99 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs (ITSMs) (2, 3). Alternate splicing generates a soluble ECD, an isoform that lacks the first Ig-like domain, and additional isoforms with deletions in the cytoplasmic domain (4). CD84 exhibits homophilic binding which is mediated by the N-terminal Ig-like domain (5). Ligation induces tyrosine phosphorylation in the cytoplasmic ITSMs which then recruit the signaling adaptor molecules SAP (SLAM‑associated protein) and EAT-2 (EWS/Fli1-activated transcript 2) (6, 7). CD84 is expressed as a 60-90 kDa molecule with extensive and cell type-specific glycosylation (2, 3, 8). It is widely expressed among hematopoietic cells including hematopoietic stem cells (8), myeloid cells (e.g. macrophages, monocytes, dendritic cells, granulocytes, and mast cells) (3, 6, 8‑10), platelets and megakaryocytes (3, 5, 8, 11), and lymphocytes. Within the T cell lineage, CD84 is expressed on thymocytes, CD4
-CD8
- cells, single positive CD4 or CD8 cells, NKT cells, and on mouse but not human NK cells (5, 6, 8, 9, 12). Within the B cell lineage, it is expressed on pro- and pre-, mature, marginal zone, and memory B cells as well as plasma cells (6, 8, 13). CD84 signaling inhibits Fc epsilon RI-induced mast cell activation (10) but enhances platelet activation (11), LPS-induced macrophage activation (8), T cell proliferation and IFN-gamma production (5, 7), and the interactions between T cells and B cells that are required for germinal center formation (14).
- Cannons, J.L. et al. (2011) Annu. Rev. Immunol. 29:665.
- de la Fuente, M.A. et al. (1997) Blood. 90:2398.
- Krause, S.W. et al. (2000) Biochem. J. 346:729.
- Palou, E. et al. (2000) Tissue Antigens 55:118.
- Martin, M. et al. (2000) J. Immunol. 167:3668.
- Tangye, S.G. et al. (2002) Eur. J. Immunol. 32:1640.
- Tangye, S.G. et al. (2003) J. Immunol. 171:2485.
- Sintes, J. et al. (2010) J. Leukoc. Biol. 88:687.
- Romero, X. et al. (2004) Tissue Antigens 64:132.
- Alvarez-Errico, D. et al. (2011) J. Immunol. 187:5577.
- Nanda, N. et al. (2005) Blood 106:3028.
- Wang, N. et al. (2010) J. Immunol. 185:5683.
- De Salort, J. et al. (2011) Immunol. Lett. 134:129.
- Cannons, J.L. et al. (2010) Immunity 32:253.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mu
Applications: ELISA, Flow, Func, WB
Species: Mu
Applications: CyTOF-ready, Flow, WB
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
Species: Hu
Applications: BA
Species: Hu
Applications: CyTOF-ready, Flow, WB
Species: Mu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, WB
Species: Hu
Applications: AgAct, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, WB
Species: Hu
Applications: CyTOF-ready, Flow
Species: Hu, Mu
Applications: Simple Western, WB
Species: Hu
Applications: Flow, IHC, IHC-P, WB
Species: Hu
Applications: B/N, Flow, IHC, IHC-Fr, WB
Species: Hu
Applications: WB
Species: Hu
Applications: B/N, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IP, WB
Species: Hu
Applications: BA
Species: Hu
Applications: Block, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, WB
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, WB
Species: Hu
Applications: Flow, IHC, IHC-P, WB
Publications for CD84/SLAMF5 (1855-CD) (0)
There are no publications for CD84/SLAMF5 (1855-CD).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for CD84/SLAMF5 (1855-CD) (0)
There are no reviews for CD84/SLAMF5 (1855-CD).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
FAQs for CD84/SLAMF5 (1855-CD) (0)
Additional CD84/SLAMF5 Products
Blogs on CD84/SLAMF5