Reactivity | HuSpecies Glossary |
Applications | Bioactivity, Enzyme Activity |
Format | Carrier-Free |
Details of Functionality | Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human CD38 His-tag binds to Human CD38 Antibody (Catalog #
AF2404) with an ED50 of 1.50-15.0 ng/mL. Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDP-ribose. Graeff, R.M. et al. (1994) J. Biol. Chem. 269:30260. The specific activity is >2200 pmol/min/μg, as measured under the described conditions. |
Source | Mouse myeloma cell line, NS0-derived human CD38 protein Val43-Ile300 with a C-terminal 6-His tag |
Accession # | |
N-terminal Sequence | Val43 |
Structure / Form | Biotinylated via amines |
Protein/Peptide Type | Recombinant Enzymes |
Endotoxin Note | <0.10 EU per 1 μg of the protein by the LAL method. |
Dilutions |
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Theoretical MW | 31 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE | 40-44 kDa, under reducing conditions |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Supplied as a 0.2 μm filtered solution in MES and NaCl. |
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Multifunctional CD38 CD38 is a 42 kD type II transmembrane glycoprotein that uses nicotinamide adenine dinucleotide (NAD) as a substrate to form cyclic adenosine diphosphate ribose (cADPR). This novel multifunctional ectoenzyme has both cyclase and hydrolase enzymatic act... Read full blog post. |
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