| Reactivity | HuSpecies Glossary |
| Applications | Bioactivity |
| Format | Carrier-Free |
| Details of Functionality | The specific activity of PLK1 was equivalent to 42 nmol/min/mg as per Activity Assay Protocol and was equivalent to 17 nmol/min/mg as per radiometric assay. |
| Source | Spodoptera frugiperda, Sf 9 (baculovirus)-derived human PLK1 protein |
| Accession # | |
| N-terminal Sequence | Using an N-terminal His tag |
| Protein/Peptide Type | Recombinant Proteins |
| Gene | PLK1 |
| Purity | >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane. |
| Dilutions |
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| SDS-PAGE | 70 kDa |
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| Publications |
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| Storage | This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
| Buffer | Supplied in 50 mM Sodium Phosphate (pH 7.0), 300 mM NaCl, 150 mM Imidazole, 0.1 mM PMSF, 0.25 mM DTT, and 25% Glycerol. |
| Purity | >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane. |
| Assay Procedure |
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PLK1 is a member of the Polo-Like Kinase family that localizes to centrosomes or spindle pole bodies and undergoes dramatic subcellular relocation during the cell cycle. Deregulated activities of PLKs often result in abnormalities in centrosome duplication, maturation, and/or microtubule dynamics (1). PLKs also regulate the function of the Golgi complex. Deregulated expression of human PLK1 is strongly correlated with the development of many types of malignancies, and ectopic expression of PLK1 dominant negative protein leads to rapid cell death (2).
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