| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | 658526 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human RCOR1/CoREST Ala394-Ser482 Accession # Q9UKL0 |
| Specificity | Detects human RCOR1/CoREST in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human RCOR3 is observed. |
| Source | N/A |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RCOR1 |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.5 mg/mL. |
RCOR1 (REST [RE-1 silencing transcription factor] CORepressor 1; also CoREST) is a 64-66 kDa member of the CoREST family of proteins. It is a nuclear protein that is ubiqui-tously expressed. RCOR1 serves as a molecular bridge, linking REST with Sin3, HDAC, LSD1, histone H3 K9 me-thyltransferases, and MeCP2. This creates a complex that represses the expression of neuron-specific genes in nonneuronal cells, principally by coordinating the demethylation of Lys4 in histone H3. Human RCOR1 is 482 amino acids (aa) in length. It contains one ELM2 domain (aa 102-157) that interacts with HDAC, and two SANT sequences (aa 187-238 and 378‑429) that bind DNA tandem repeats. There are three potential RCOR1 splice vari-ants. One contains a four aa substitution for aa 296-482, a second shows an alternative start site 3 aa upstream of the standard site, while a third shares the same upstream start site accompanied by a 15 aa substitution for aa 471-482. Over aa 394-482, human RCOR1 shares 93% aa identity with mouse RCOR1.
Secondary Antibodies |
Isotype Controls |
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![Western Blot G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-4120241252118.jpg)
![Immunohistochemistry G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-412024125214.jpg)
![Immunohistochemistry G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-4120241251265.jpg)
