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RBX1 Antibody (1076128) [Unconjugated]

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Western Blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human RBX1 Monoclonal Antibody (Catalog # MAB11499) followed by HRP-conjugated ...read more
RBX1 was detected in immersion fixed paraffin-embedded sections of prostate tumor using Mouse Anti-Human RBX1 Monoclonal Antibody (Catalog # MAB11499) at 5 µg/ml for 1 hour at room temperature followed by incubation ...read more
RBX1 was detected in fixed MCF‑7 human breast cancer cell line (Positive) and absent in Jurkat human acute T cell leukemia cell line (Negative) using Mouse Anti-Human RBX1 Monoclonal Antibody (Catalog # MAB11499) ...read more
Simple Western shows lysates of HEK293T human embryonic kidney cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for RBX1 at approximately 19 kDa (as ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clone
1076128
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price
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RBX1 Antibody (1076128) [Unconjugated] Summary

Immunogen
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL1/RBX1 Complex
Met1-His108
Accession # P62877
Specificity
Detects human RBX1 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Simple Western 100 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RBX1 Antibody (1076128) [Unconjugated]

  • BA554C12.1
  • EC 6.3.2.-
  • FLJ60363
  • MGC13357
  • Protein ZYP
  • RBX1
  • Regulator of cullins 1
  • Ring Box 1
  • RING box protein 1
  • RING finger protein 75
  • ring-box 1
  • ring-box 1, E3 ubiquitin protein ligase
  • RING-box protein 1
  • RNF75
  • RNF75MGC1481
  • ROC1E3 ubiquitin-protein ligase RBX1
  • ZYP Protein

Background

RBX1 is a constitutively expressed RING-box protein that interacts with cullins. The human RBX1 gene consists of 5 exons and 4 introns and encodes 108 amino acids with a molecular weight of about 14kda. It is a major component of E3 ubiquitin ligase. A characteristic feature of RBX1 structure is that it contains a RING-H2 finger domain at the carboxyl terminus.  RBX1 is overexpressed in many primary tumors and is associated with overall survival and lymph node metastasis. In humans, RBX1 has high expression in the heart, skeletal muscle, and testis with less expression in the brain, lung, kidney, and placenta.
  1. Shao J, Feng Q, Jiang W, Yang Y, Liu Z, Li L, Yang W, Zou Y. E3 ubiquitin ligase RBX1 drives the metastasis of triple negative breast cancer through a FBXO45-TWIST1-dependent degradation mechanism. Aging (Albany NY). 2022 Jul 8;14(13):5493-5510. doi: 10.18632/aging.204163. Epub 2022 Jul 8. PMID: 35802537; PMCID: PMC9320552. 
  2. Wei D, Sun Y. Small RING Finger Proteins RBX1 and RBX2 of SCF E3 Ubiquitin Ligases: The Role in Cancer and as Cancer Targets. Genes Cancer. 2010 Jul;1(7):700-7. doi: 10.1177/1947601910382776. PMID: 21103004; PMCID: PMC2983490.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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