RBFOX3/NeuN Antibody - BSA Free

Rbfox3 (also known as Fox-3, Fox1 homolog C, Hrnbp3, and D11Bwg0517e) is one of three mammalian members of the RNA-binding protein Rbfox gene family, all of which are involved in regulating alternative RNA splicing. Originally identified as an epitope, NeuN is located within the N-terminal region (6-15 amino acids) of Rbfox3. Rbfox family members are highly conserved, having a single RNA recognition motif (RRM)-type RNA binding domain (RBD) near the center of the protein sequence. The RBD amino acid (aa) sequence is identical between members, Rbfox1 and Rbfox2, with 4 differences within the 77 aa domain of Rbfox3. The fox3 gene is comprised of 15 exons in humans, 11 exons in rat, with 3 variants in mouse containing 14 exons and another 3 variants containing 15 exons. The Rbfox3 protein is highly conserved across human, mouse, and rat with 98.9% sequence identity between mouse (isoform I) and rat and 83.9% sequence identity between mouse (isoform I) and human. The expression of Rbfox3/NeuN is restricted to the nervous system and has widely been used in stroke research. It is recognized as a marker of mature neuronal cell types in the spinal cord, cerebral cortex, hippocampus, dorsal thalamus, caudate/putamen, and cerebellum. NeuN has been shown to bind DNA and is predominantly nuclear. Differences in immunoreactivity have been reported between Rbfox3/NeuN subtypes in which the 46kDa is mainly found in the nucleus whereas the 48kDa form is primarily distributed in the cytoplasm (1,2).

Rbfox proteins participate in regulation of alternative splicing between family members as well as in autoregulation. While the breadth of brain and muscle specific targets for alternative splicing is well established for Rbfox proteins, those specific to Rbfox3/NeuN along with its alternative splicing mechanism are less understood. Rbfox3 has been shown to regulate neuronal differentiation by alternative splicing of Numb pre-mRNA and has a role in adult neurogenesis. Dysfunctional Rbfox3/NeuN has been associated with various neurological disorders such as neurodevelopmental delay, autism spectrum disorder, Benign rolandic epilepsy (BRE), and cognitive impairments (1,2).

References

1. Duan W, Zhang YP, Hou Z, Huang C, Zhu H, Zhang CQ, Yin Q. (2016) Novel Insights into NeuN: from Neuronal Marker to Splicing Regulator. Mol Neurobiol. 53(3):1637-1647. PMID: 25680637.

2. Su CH, D D, Tarn WY. (2018) Alternative Splicing in Neurogenesis and Brain Development. Front Mol Biosci. 5:12. PMID: 29484299

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-FrFl, IHC-P, Immunohistochemistry, WB.

Showing Publications 1 - 10 of 31. Show All 31 Publications. Collapse Publications.

Publications using NBP1-77686	Applications	Species
Li F, Liu Y, Li L et al. Brain-derived extracellular vesicles mediate traumatic brain injury associated multi-organ damage Biochemical and Biophysical Research Communications 2023-05-01 [PMID: 37163934] (Mouse)		Mouse
Issler O, van der Zee YY, Ramakrishnan A et al. The long noncoding RNA FEDORA is a cell type- and sex-specific regulator of depression Science Advances 2022-12-02 [PMID: 36449610] (Immunohistochemistry)	Immunohistochemistry
Bogdanov L, Shishkova D, Mukhamadiyarov R et al. Excessive Adventitial and Perivascular Vascularisation Correlates with Vascular Inflammation and Intimal Hyperplasia International journal of molecular sciences 2022-10-12 [PMID: 36293013] (IF/IHC, Rat) Details: Dilution used in IHC 1:100	IF/IHC	Rat
Stewart A, Glaser E, Mott CA et al. Advanced Age and Neurotrauma Diminish Glutathione and Impair Antioxidant Defense after Spinal Cord Injury Journal of neurotrauma 2022-04-04 [PMID: 35373589] (IF/IHC, Mouse)	IF/IHC	Mouse
Yang C, Lavayen BP, Liu L Et al. Neurovascular protection by adropin in experimental ischemic stroke through an endothelial nitric oxide synthase-dependent mechanism Redox biology 2021-11-22 [PMID: 34826783] (IF/IHC, Mouse)	IF/IHC	Mouse
Kopper TJ Myelin, cPLA2, and Azithromycin: Modulation of Macrophage Activation in Spinal Cord Injury Inflammation Thesis 2021-01-01 (IF/IHC)	IF/IHC
MendonCa IP, Paiva IHR, Duarte-Silva EP et al. Metformin and fluoxetine improve depressive-like behavior in a murine model of Parkinsons disease through the modulation of neuroinflammation, neurogenesis and neuroplasticity International immunopharmacology 2022-01-01 [PMID: 34890997] (IF/IHC, Mouse)	IF/IHC	Mouse
Wang W, Cao Q, Tan T et al. Epigenetic treatment of behavioral and physiological deficits in a tauopathy mouse model Aging Cell 2021-10-01 [PMID: 34547169] (Immunohistochemistry)	Immunohistochemistry
Williams JB, Cao Q, Yan Z. Transcriptomic analysis of human brains with Alzheimer's disease reveals the altered expression of synaptic genes linked to cognitive deficits Brain Communications 2021-07-01 [PMID: 34423299] (Immunohistochemistry)	Immunohistochemistry
Chen X, Jiang M, Li H et al. CX3CL1/CX3CR1 axis attenuates early brain injury via promoting the delivery of exosomal microRNA-124 from neuron to microglia after subarachnoid hemorrhage J Neuroinflammation 2020-07-14 [PMID: 32664984] (ICC/IF, Human, Rat)	ICC/IF	Human, Rat
Williams JB Transcriptomic and Epigenomic Analysis Reveals Convergent Synaptic Deficits in Alzheimer's Disease Thesis 2022-01-01 (IF/IHC, Human)	IF/IHC	Human
Wilson CS, Dohare P, Orbeta S et al. Late Adolescence Mortality in Mice with Brain-Specific Deletion of the Volume-Regulated Anion Channel Subunit LRRC8A FASEB J 2021-09-01 [PMID: 34469026]
Arvola O, Griffiths B, Rao A Et Al. Expression of miR-200c corresponds with increased reactive oxygen species and hypoxia markers after transient focal ischemia in mice Neurochemistry international 2021-07-31 [PMID: 34343653] (IHC-FrFl)	IHC-FrFl
Ho Mh, Yen Ch, Hsieh Th Et Al. CCL5 via GPX1 activation protects hippocampal memory function after mild traumatic brain injury Redox biology 2021-07-17 [PMID: 34315111] (IF/IHC)	IF/IHC
Chaudhary S, Ashok A, Wise AS et al. Upregulation of brain hepcidin in prion diseases Prion 2021-12-01 [PMID: 34224321] (ICC/IF)	ICC/IF
Stewart AN, McFarlane KE, Vekaria HJ, et al. Mitochondria exert age-divergent effects on recovery from spinal cord injury Experimental neurology 2021-01-07 [PMID: 33422552] (IF/IHC, Mouse)	IF/IHC	Mouse
Douglas AS, Shearer JA, Okolo A et al. The Relationship Between Cerebral Reperfusion And Regional Expression Of Matrix Metalloproteinase-9 In Rat Brain Following Focal Cerebral Ischemia Neuroscience 2020-11-18 [PMID: 33220187] (ICC/IF, Rat) Details: Immunofluorescence staining brain of rats who underwent Middle Cerebral Artery Occlusion	ICC/IF	Rat
Kopper TJ, McFarlane KE, Bailey WM et al. Delayed Azithromycin Treatment Improves Recovery After Mouse Spinal Cord Injury Front Cell Neurosci 2019-11-06 [PMID: 31780896] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Sipos E NEUROPATHOLOGICAL EXAMINATIONS IN THE MOUSE BRAININVESTIGATION OF A NEW PHARMACEUTICAL TARGET IN THE CUPRIZONE MODEL AND COMPARATIVE HISTOLOGICAL ANALYSES OF PRIMARY CILIA IN THE PHYSIOLOGICAL CENTRAL NERVOUS SYSTEM Thesis 2020-01-01 (IHC-Fr, Mouse)	IHC-Fr	Mouse
Taurine/Pilocarpine Interaction in the Malnourished Rat Brain: A Behavioral, Electrophysiological, and Immunohistochemical Analysis Front. Francisco E, Mendes-da-Silva R, Castro C et al. Front Neurosci [PMID: 31619952] (IHC-Fr, Rat)	IHC-Fr	Rat
Stefani FR, Eberstal S, Vergani S et al. Low-dose irradiated mesenchymal stromal cells break tumor defensive properties in vivo Int. J. Cancer 2018-05-11 [PMID: 29752716] (IF/IHC, Mouse)	IF/IHC	Mouse
Holmes A, Zhou N, Donahue DL et al. A deficiency of the GluN2C subunit of the N-methyl-D-aspartate receptor is neuroprotective in a mouse model of ischemic stroke Biochem. Biophys. Res. Commun. 2017-10-31 [PMID: 29101031] (Mouse)		Mouse
DeMars KM, Pacheco SC, Yang C et al. Selective Inhibition of Janus Kinase 3 Has No Impact on Infarct Size or Neurobehavioral Outcomes in Permanent Ischemic Stroke in Mice. Front Neurol 2017-08-09 [PMID: 28790974] (ICC/IF, Mouse)	ICC/IF	Mouse
Zhang B, Bailey WM, McVicar AL, Gensel JC. Age increases reactive oxygen species production in macrophages and potentiates oxidative damage after spinal cord injury. Neurobiol Aging 2016-11-01 [PMID: 27596335]
Kim BW, Jeong KH, Kim JH et al. Pathogenic Upregulation of Glial Lipocalin-2 in the Parkinsonian Dopaminergic System. J. Neurosci. 2016-05-18 [PMID: 27194339] (IF/IHC, Mouse)	IF/IHC	Mouse
Yin Q, Fan Q, Zhao Y et al. Spinal NF-kB and Chemokine Ligand 5 Expression during Spinal Glial Cell Activation in a Neuropathic Pain Model PLoS ONE. 2015-01-31 [PMID: 25635831] (IHC-Fr, Rat) Details: RBFOX3/NeuN antibody used for IHC-Fr on L4-5 spinal segments sections of Rats subjected or not to chronic constriction injury/CCI-induced neuropathic pain model. The immunostaining assay involved - frozen longitudinal slices of 20-30 um thickness, non-specific IgG blocking with 10% donkey serum in PBS, use of NeuN antibody at 1:600 dilution with ON 4C incubation, detection with IF labelled secondary antibody, addition of cover-slips to sectiions with a mixture of 50% glycerin in 0.01 M PBS for analysis under microscope. Neun studies in reference to co-localization with NFkB and CCL5 proteins and the data is shown in Figure 7.	IHC-Fr	Rat
de Paiva I, Silva R, Mendonça I et al. Semaglutide Attenuates Anxious And Depressive-Like Behaviors and Reverses The Cognitive Impairment in a Type 2 Diabetes Mellitus Via The Microbiota-Gut-Brain Axis Research Square 2023-09-15 (IHC-P, Mouse)	IHC-P	Mouse
Robinson M, Chapani P, Styan T et al. Functionalizing Ascl1 with Novel Intracellular Protein Delivery Technology for Promoting Neuronal Differentiation of Human Induced Pluripotent Stem Cells. Stem Cell Rev. 2016-05-02 [PMID: 27138845] (ICC/IF, Human) Details: This publication used the AF405 conjugated form of this antibody (NBP1-77686AF405)	ICC/IF	Human
Hu A, Yuan H, Wu L et al. The effect of constitutive over-expression of insulin-like growth factor 1 on the cognitive function in aged mice. Brain Res. 2015-11-12 [PMID: 26581336] (WB, IHC-Fr, Mouse) Details: RBFOX3/NeuN antibody was used at 1:500 dilution for WB application on lysates of brains from aged IGF-1 transgenic mice versus the wild-type C57BL/6J mice. The antibody was used for IHC-Fr application also and the assay implicated - fixation of brains in 4% paraformaldehyde/4C ON followed by 30% sucrose, 25um sections, blocking with10% normal goat serum, use of primary at 1:400 dilution, detection with FITC conjugated secondary (Fig. 3)	WB, IHC-Fr	Mouse
Zou H, Feng R, Huang Y et al. Double minute amplification of mutant PDGF receptor alpha in a mouse glioma model. Sci Rep 2015-01-01 [PMID: 25683249] (IF/IHC, Mouse)	IF/IHC	Mouse
Samano C, Nasrabady SE, Nistri A. A study of the potential neuroprotective effect of riluzole on locomotor networks of the neonatal rat spinal cord in vitro damaged by excitotoxicity Neuroscience 2012-07-04 [PMID: 22771622] (ICC/IF, IF/IHC, Rat)	ICC/IF, IF/IHC	Rat
Show All 31 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	WB	Rat	07/28/2020	View Summary Application Western Blot Sample Tested retina Species Rat Lot C-1
Enlarge	4 reviewed by: Verified Customer	WB	Mouse	07/28/2020	View Summary Application Western Blot Sample Tested retina Species Mouse Lot C-1
Enlarge	3 reviewed by: Suman Chaudhary	Simple Western	Human	07/21/2020	View Summary Application Simple Western Sample Tested Adult eye and muscle tissue (labeled E and M Species Human Lot C-1
Enlarge	5 reviewed by: Verified Customer	WB	Bovine	07/21/2020	View Summary Application Western Blot Sample Tested retina Species Bovine Lot C-1
Enlarge	5 reviewed by: Ajay Ashok	WB	Pig	07/21/2020	View Summary Application Western Blot Sample Tested retina Species Pig Lot C-1
Enlarge	5 reviewed by: Verified Customer	WB	Goat	07/21/2020	View Summary Application Western Blot Sample Tested retina Species Goat Lot C-1
Enlarge	5 reviewed by: Verified Customer	ICC	Human	06/23/2020	View Summary Application Immunocytochemistry Sample Tested mixed primary neuronal culture Species Human Lot C1

Array NBP1-77686

• RBFOX3/NeuN Antibodies
• RBFOX3/NeuN Lysate
• RBFOX3/NeuN Proteins