NeuN is a RNA-binding protein that modulates alternative splicing and is localized both to the nucleus and cytoplasm. It is a member of the RNA-binding FOX (RBFOX) family of splicing regulators which includes RBFOX1 (Fox-1/A2BP1) and RBFOX2 (Fox-2/RBM9). Each FOX family member is differentially expressed – RBFOX1 in neurons, heart, and muscle while RBFOX3 is exclusively limited to neurons. RBFOX2 has a broader profile that includes the earlier mentioned tissues as well as in embryos, hematopoietic cells, and embryonic stem cells. All FOX proteins have one RNA-binding recognition domain that binds the (U)GCAUG sequence found within introns flanking alternative exons and initiates splicing.
Immunohistochemistry: RBFOX3/NeuN Antibody
The RBFOX3 antibody was used in assessments of olfactory bulb neuron age and postnatal neurogenesis and plasticity in human brains compared to other mammals (1). Samano’s group in Italy used RBFOX3 antibody in their in vivo acute rat spinal cord injury (SCI) studies to study the effects of the drug riluzole on preventing neuronal loss and protect locomotor activity (2). Recent studies with the RBFOX3 antibody in familial amyotrophic lateral sclerosis enabled profiling of neurodegeneration and axon-autonomous effects of pathogenic mutations in the superoxide dismutase 1 pathway (3).
Novus Biologicals offers RBFOX3/NeuN reagents for your research needs including:
