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RB1 Antibody

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Western Blot: RB1 Antibody [NB100-56888] - Analysis of Retinoblastoma 1 in 30 ugs of Jurkat cell lysate using NB100-56888 at 1:1000 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, EqSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

RB1 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 251-268 (RTPRRGQNRSARIAKQLE) of human Rb was used as immunogen
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:1000
Application Notes
In Jurkat, a 110 kDa band is observed.
Control
Jurkat Whole Cell Lysate
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-56888 in the following application:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for RB1 Antibody

  • OSRC
  • osteosarcoma
  • p105-Rb
  • pp110
  • pRb
  • RB
  • RB1
  • retinoblastoma 1
  • retinoblastoma suspectibility protein
  • retinoblastoma-associated protein

Background

Mutation or deregulation of Rb, retinoblastoma tumor suppressor gene, is seen so frequently in tumor cells that it is believe that some type of suppression of the Rb gene is required for cancer cell genesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RB1 Antibody (NB100-56888) (0)

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Reviews for RB1 Antibody (NB100-56888) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-56888:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Western Blot RB1 NB100-56888
Enlarge
4
reviewed by:
Verified Customer
WB Human 07/31/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell extract form Jurkat cells
SpeciesHuman
Western Blot RB1 NB100-56888
Enlarge
4
reviewed by:
Verified Customer
WB Human 07/25/2014
View

Summary

ApplicationWestern Blot
Sample TestedCell extract from 293T cells
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RB1 Antibody (NB100-56888) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional RB1 Products

Research Areas for RB1 Antibody (NB100-56888)

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Blogs on RB1

There are no specific blogs for RB1, but you can read our latest blog posts.
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Contact Information

Product PDFs

Calculators

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Verified Customer
07/31/2014
Application: WB
Species: Human

Verified Customer
07/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol RB1
Entrez
Uniprot