RB1 Antibodies
We offer RB1 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot. Each RB1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our RB1 Antibodies can be used in a variety of model species: Canine, Equine, Human, Mouse, Rat. Use the list below to choose the RB1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our RB1 polyclonal antibodies and browse our RB1 monoclonal antibody catalog.
Alternate Names for RB1 Antibodies
anti-RB1 antibody, anti-OSRC antibody, anti-osteosarcoma antibody, anti-p105-Rb antibody, anti-pp110 antibody, anti-pRb antibody, anti-RB antibody, anti-retinoblastoma 1 antibody, anti-retinoblastoma suspectibility protein antibody, anti-retinoblastoma-associated protein antibody
22 Results for "rb1" in Primary Antibodies
![Knockdown Validated: RB1 Antibody (172C1094) - (cleaved) [NB100-56598] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/RB1-Antibody-172C1094-cleaved-Knockdown-Validated-NB100-56598-img0006.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: RB1 Antibody (172C1094) - (cleaved) [NB100-56598] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in HL60 cells were treated with camptothecin for 24 hours.](http://images.novusbio.com/fullsize/cleaved-Retinoblastoma-1-Antibody-172C1094-Western-Blot-NB100-56598-img0005.jpg)
![Immunohistochemistry: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in sections of primary human breast tumors. Image courtesy of Dr. Macleod, The Ben May Institute for Cancer Research, and The University of Chicago.](http://images.novusbio.com/fullsize/cleaved-Retinoblastoma-1-Antibody-172C1094-Immunohistochemistry-NB100-56598-img0004.jpg)
![Western Blot: RB1 Antibody [NB100-56888] - Analysis of Retinoblastoma 1 in 30 ugs of Jurkat cell lysate using NB100-56888 at 1:1000 dilution.](http://images.novusbio.com/fullsize/RB1-Antibody-Western-Blot-NB100-56888-img0003.jpg "Western Blot: RB1 Antibody [NB100-56888] - Analysis of Retinoblastoma 1 in 30 ugs of Jurkat cell lysate using NB100-56888 at 1:1000 dilution.")
![Western Blot: RB1 Antibody [NBP2-20127] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rb antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RB1-Antibody-Western-Blot-NBP2-20127-img0008.jpg "Western Blot: RB1 Antibody [NBP2-20127] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rb antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: RB1 Antibody [NBP2-20127] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rb stained by Rb antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.](http://images.novusbio.com/fullsize/RB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20127-img0007.jpg)
![Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127] - Mouse eye. Green: Rb stained by Rb antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/RB1-Antibody-Immunohistochemistry-Paraffin-NBP2-20127-img0006.jpg)
![Western Blot: RB1 Antibody [NBP2-20127] - Rb antibody detects Rb1 protein by western blot analysis. A. 30 ug A431 whole cell lysate/extract 5% SDS-PAGE Rb1 antibody dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RB1-Antibody-Western-Blot-NBP2-20127-img0002.jpg)
![Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127] - Human colon carcinoma. Rb antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/RB1-Antibody-Immunohistochemistry-Paraffin-NBP2-20127-img0005.jpg)
![Immunoprecipitation: RB1 Antibody [NBP2-20127] - HeLa whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of retinoblastoma 1 protein by 4 ug Rb1 antibody 7.5 % SDS-PAGE The immunoprecipitated retinoblastoma 1 protein was detected by Rb1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/RB1-Antibody-Immunoprecipitation-NBP2-20127-img0004.jpg)
![Immunohistochemistry-Paraffin: RB1 Antibody (RB1/1754) [NBP2-53400] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (RB1/1754).](http://images.novusbio.com/fullsize/RB1-Antibody-RB1-1754-Immunohistochemistry-Paraffin-NBP2-53400-img0002.jpg "Immunohistochemistry-Paraffin: RB1 Antibody (RB1/1754) [NBP2-53400] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (RB1/1754).")
![Immunohistochemistry-Paraffin: RB1 Antibody (RB1/1754) [NBP2-53400] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (RB1/1754).](http://images.novusbio.com/fullsize/RB1-Antibody-RB1-1754-Immunohistochemistry-Paraffin-NBP2-53400-img0001.jpg)
![Western Blot: RB1 [p Ser795] Antibody [NB100-82176] - Analysis of RB1 [p Ser795] ( using NB100-82176 in K562 cells untreated or treated with 10% serum after 48 hours of starvation.](http://images.novusbio.com/fullsize/RB1-[p-Ser795]-Antibody-Western-Blot-NB100-82176-img0013.jpg "Western Blot: RB1 [p Ser795] Antibody [NB100-82176] - Analysis of RB1 [p Ser795] ( using NB100-82176 in K562 cells untreated or treated with 10% serum after 48 hours of starvation.")
![Immunocytochemistry/Immunofluorescence: RB1 [p Ser795] Antibody [NB100-82176] - Staining of methanol-fixed Hela cells using Rb1 (P Ser795) antibody](http://images.novusbio.com/fullsize/RB1-[p-Ser795]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82176-img0011.jpg)
![Immunohistochemistry-Paraffin: RB1 [p Ser795] Antibody [NB100-82176] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-82176 (left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/RB1-[p-Ser795]-Antibody-Immunohistochemistry-NB100-82176-img0009.jpg)
![Immunohistochemistry-Paraffin: RB1 Antibody (1F8) [NBP2-53398] - Formalin-fixed, paraffin-embedded human Colon stained with RB1 Antibody (1F8).](http://images.novusbio.com/fullsize/RB1-Antibody-1F8-Immunohistochemistry-Paraffin-NBP2-53398-img0002.jpg "Immunohistochemistry-Paraffin: RB1 Antibody (1F8) [NBP2-53398] - Formalin-fixed, paraffin-embedded human Colon stained with RB1 Antibody (1F8).")
![Immunohistochemistry-Paraffin: RB1 Antibody (SPM353) [NBP2-53399] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (SPM353).](http://images.novusbio.com/fullsize/RB1-Antibody-SPM353-Immunohistochemistry-Paraffin-NBP2-53399-img0001.jpg "Immunohistochemistry-Paraffin: RB1 Antibody (SPM353) [NBP2-53399] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (SPM353).")
![Western Blot: RB1 [p Ser807] Antibody [NB100-82177] - Analysis of extracts from 3T3 cells untreated or treated with UV using Rb(Phospho-Ser807) Antibody](http://images.novusbio.com/fullsize/RB1-[p-Ser807]-Antibody-Western-Blot-NB100-82177-img0006.jpg "Western Blot: RB1 [p Ser807] Antibody [NB100-82177] - Analysis of extracts from 3T3 cells untreated or treated with UV using Rb(Phospho-Ser807) Antibody")
![Immunohistochemistry-Paraffin: RB1 [p Ser807] Antibody [NB100-82177] - Analysis of paraffin-embedded human breast carcinoma tissue using NB100-82177 (left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/RB1-[p-Ser807]-Antibody-Immunohistochemistry-NB100-82177-img0005.jpg)
![Western Blot: RB1 Antibody (S09-4H8) [NBP3-19971] - Western blot detection of RB1 in 3T3 cell lysates using NBP3-19971 (1:1000 diluted). Predicted band size: 106kDa. Observed band size: 110kDa.](http://images.novusbio.com/fullsize/RB1-Antibody-S09-4H8-Western-Blot-NBP3-19971-img0002.jpg "Western Blot: RB1 Antibody (S09-4H8) [NBP3-19971] - Western blot detection of RB1 in 3T3 cell lysates using NBP3-19971 (1:1000 diluted). Predicted band size: 106kDa. Observed band size: 110kDa.")
![Immunocytochemistry/Immunofluorescence: RB1 Antibody (S09-4H8) [NBP3-19971] - Immunocytochemistry of RB1 (green) in Hela cells using NBP3-19971 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/RB1-Antibody-S09-4H8-Immunocytochemistry-Immunofluorescence-NBP3-19971-img0001.jpg)
![Western Blot: RB1 [p Ser811] Antibody (S01-2G6) [NBP3-19969] - Western blot detection of RB1 in Hela (Lane 1), CHO-K1 (Lane 2) cell lysates using NBP3-19969 (1:500 diluted). Predicted band size: 106kDa. Observed band size: 110kDa.](http://images.novusbio.com/fullsize/RB1-p-Ser811-Antibody-S01-2G6-Western-Blot-NBP3-19969-img0001.jpg "Western Blot: RB1 [p Ser811] Antibody (S01-2G6) [NBP3-19969] - Western blot detection of RB1 in Hela (Lane 1), CHO-K1 (Lane 2) cell lysates using NBP3-19969 (1:500 diluted). Predicted band size: 106kDa. Observed band size: 110kDa.")