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RASD2 Antibody

Images

 
Western Blot: RASD2 Antibody [NBP1-82775] - Analysis in control (vector only transfected HEK293T lysate) and RASD2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Orthogonal Strategies: Immunohistochemistry-Paraffin: RASD2 Antibody [NBP1-82775] - Staining in human prostate and pancreas tissues using anti-RASD2 antibody. Corresponding RASD2 RNA-seq data are presented for ...read more
Immunohistochemistry-Paraffin: RASD2 Antibody [NBP1-82775] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: RASD2 Antibody [NBP1-82775] - Staining of human prostate shows high expression.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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RASD2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: DTSGNHPFPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTKEAAELPMVICGNKNDHGELCRQVPTTEAELLVSGDENCAYFEVSAKKNTNVDEMFYVLFSMAKLPHEMSPALHRKISVQYGDAFHPRPFCMRRVKEMDAYGMVSPFARRPSVNSDLKYIKAKVLR
Predicted Species
Mouse (94%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RASD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Western Blot verified by a customer review.
Control Peptide
RASD2 Protein (NBP1-82775PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-82775 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RASD2 Antibody

  • GTP-binding protein Rhes
  • MGC:4834
  • RASD family, member 2
  • Rhes
  • TEM2Ras homolog enriched in striatum
  • Tumor endothelial marker 2

Background

RASD2 (RASD Family, Member 2) is involved in GTPase activity. While the exact function of RASD2 has yet to be determined, it is thought to have a role in signal transduction. RASD2 is known to have interactions with ACVR1, SMAD2, SMAD3, SMURF1 and PIK3CA. RASD2 has been studied in relation to Huntington's disease and pancreatitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RASD2 Antibody (NBP1-82775) (0)

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Review for RASD2 Antibody (NBP1-82775) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse and Rat.

Reviews using NBP1-82775:
Filter by Applications
WB
(1)
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Filter by Species
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(1)
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Images Ratings Applications Species Date Details
Western Blot RASD2 NBP1-82775
Enlarge
5
reviewed by:
Verified Customer
WB Mouse and Rat 05/03/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse Neuro2A whole cell lysate,PC12 cell whole lysate, Sample Amount: 25ug
SpeciesMouse and Rat

Comments

CommentsAnother company's RASD2 antibody displayed numerous 'zebra stripes', we stripped and reprobed this membrane with this Novus antibody and were able to detect endogenous RASD2 in the PC12 cells, and not in the N2A cells as they don't express RASD2.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RASD2 Antibody (NBP1-82775) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on RASD2

There are no specific blogs for RASD2, but you can read our latest blog posts.
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Calculators

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Recent Reviews

5
5
1
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1
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Verified Customer
05/03/2017
Application: WB
Species: Mouse and Rat

Bioinformatics

Gene Symbol RASD2