![Radixin Antibody (1E12) [H00005962-M01] - (A) Western blot analysis of radixin levels in SKBR3 and HeLa cells. (B) Colocalization of radixin and ErbB2 in SKBR3 cells. 3D-SIM of fixed cells, stained for endogenous ERM and ErbB2, shows a high degree of colocalization between the radixin and ErbB2 at the plasma membrane (left panel: max. projection; middle: single plane section; right: single channels of insert). Scale bars: 10 um. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.8327) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Radixin-Antibody-1E12-H00005962-M01-img0006.jpg "Radixin Antibody (1E12) [H00005962-M01] - (A) Western blot analysis of radixin levels in SKBR3 and HeLa cells. (B) Colocalization of radixin and ErbB2 in SKBR3 cells. 3D-SIM of fixed cells, stained for endogenous ERM and ErbB2, shows a high degree of colocalization between the radixin and ErbB2 at the plasma membrane (left panel: max. projection; middle: single plane section; right: single channels of insert). Scale bars: 10 um. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.8327) licensed under a CC-BY license.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 1E12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | RDX (AAH47109, 1 a.a. ~ 583 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTVGLREVWFFGLQYVDSKGYSTWLKLNKKVTQQDVKKENPLQFKFRAKFFPEDVSEELIQEITQRLFFLQVKEAILNDEIYCPPETAVLLASYAVQAKYGDYNKEIHKPGYLANDRLLPQRVLEQHKLTKEQWEERIQNWHEEHRGMLREDSMMEYLKIAQDLEMYGVNYFEIKNKKGTELWLGVDALGLNIYEHDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILALCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERAQLENEKEKREIAEKEKERIEREKEELMERLKQIEEQTIKAQKELEEQTRKALELDQERKRAKEEAERLEKERRAAEEAKSAIAKQAADQMKNQEQLAAELAEFTAKIALLEEAKKKKEEEATEWQHKAFAAQEDLEKTKEELKTVMSAPPPPPPPPVIPPTENEHDEHDENNAEASAELSNEGVMNHRSEEERVTETQKNERVKKQLQALSSELAQARDETKKTQNDVLHAENVKAGRDKYKTLRQIRQGNTKQRIDEFEAM |
| Specificity | RDX - radixin (1E12) |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RDX |
| Purity | Ascites |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been use for IF. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | Ascites |
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| Publication using H00005962-M01 | Applications | Species |
|---|---|---|
| Asp N, Kvalvaag A, Sandvig K, Pust S. Regulation of ErbB2 localization and function in breast cancer cells by ERM proteins. Oncotarget. 2016-05-03 [PMID: 27029001] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Radixin Antibody (H00005962-M01)Find related products by research area.
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![Western Blot: Radixin Antibody (1E12) [H00005962-M01] - RDX monoclonal antibody (M01), clone 1E12. Analysis of RDX expression in human kidney.](http://images.novusbio.com/fullsize/Radixin-Antibody-1E12-Western-Blot-H00005962-M01-img0005.jpg "Western Blot: Radixin Antibody (1E12) [H00005962-M01] - RDX monoclonal antibody (M01), clone 1E12. Analysis of RDX expression in human kidney.")
![Immunocytochemistry/Immunofluorescence: Radixin Antibody (1E12) [H00005962-M01] - Analysis of monoclonal antibody to RDX on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Radixin-Antibody-1E12-Immunocytochemistry-Immunofluorescence-H00005962-M01-img0004.jpg "Immunocytochemistry/Immunofluorescence: Radixin Antibody (1E12) [H00005962-M01] - Analysis of monoclonal antibody to RDX on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: Radixin Antibody (1E12) [H00005962-M01] - Localization studies of ERM proteins, Ebp50 & ErbB2 in SKBR3 cells & protein proximity analysis(A) Confocal microscopy of ERM proteins & Ebp50. Untreated cells were fixed, permeabilized & stained by specific antibodies for endogenous levels of ezrin, radixin & Ebp50 (max. projection). In SKBR3 cells ezrin & radixin strongly colocalizes with Ebp50 at the plasma membrane. (B) Confocal microscopy (single plane section) & (C) 3D-SIM (max. projections) of ErbB2 & Ebp50 localization. In untreated control conditions, ErbB2 shows a strong colocalization with Ebp50 in lamellipodia-like structures at the plasma membrane. (D-F) Analysis of protein proximity by PLA experiments. SKBR3 cells were transfected either with non-targeting control siRNA or ezrin/radixin specific siRNA. Cells were fixed 72 h after transfection & PLA experiments were performed. The depletion of ezrin or radixin strongly reduces the interaction of ErbB2 with Ebp50 (D), Hsp90 (E) & ErbB3 (F). All results are shown as mean +/− SEM (*P < 0.05; ***P < 0.001). Scale bars: 10 μm (A, B), 8 μm (C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27029001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005962-m01_mouse-monoclonal-radixin-antibody-1e12-310202415482223.jpg "Immunocytochemistry/ Immunofluorescence: Radixin Antibody (1E12) [H00005962-M01] - Localization studies of ERM proteins, Ebp50 & ErbB2 in SKBR3 cells & protein proximity analysis(A) Confocal microscopy of ERM proteins & Ebp50. Untreated cells were fixed, permeabilized & stained by specific antibodies for endogenous levels of ezrin, radixin & Ebp50 (max. projection). In SKBR3 cells ezrin & radixin strongly colocalizes with Ebp50 at the plasma membrane. (B) Confocal microscopy (single plane section) & (C) 3D-SIM (max. projections) of ErbB2 & Ebp50 localization. In untreated control conditions, ErbB2 shows a strong colocalization with Ebp50 in lamellipodia-like structures at the plasma membrane. (D-F) Analysis of protein proximity by PLA experiments. SKBR3 cells were transfected either with non-targeting control siRNA or ezrin/radixin specific siRNA. Cells were fixed 72 h after transfection & PLA experiments were performed. The depletion of ezrin or radixin strongly reduces the interaction of ErbB2 with Ebp50 (D), Hsp90 (E) & ErbB3 (F). All results are shown as mean +/− SEM (*P < 0.05; ***P < 0.001). Scale bars: 10 μm (A, B), 8 μm (C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27029001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Radixin Antibody (1E12) [H00005962-M01] - ERM expression & localization in SKBR3 breast cancer cells(A) Western blot analysis of ERM levels in SKBR3, HeLa & PC3 cells. SKBR3 breast cancer cells do not express moesin. (B) Colocalization of ezrin/radixin & ErbB2 in SKBR3 cells. 3D-SIM of fixed cells, stained for endogenous ERM & ErbB2, shows a high degree of colocalization between the ezrin/radixin & ErbB2 at the plasma membrane (left panel: max. projection; middle: single plane section; right: single channels of insert). Scale bars: 10 μm. (C) Analysis of protein association in SKBR3 cells by proximity ligation assay (PLA). 2 h treatment with 3 μM GA leads to decreased association of ezrin/ErbB2 & radixin/ErbB2. Data is represented as mean +/− SEM (***P < 0.001). (D) Corresponding single plan section of a representative PLA experiment. Fluorescence & DIC pictures of control cells (upper panel) & cell treated for 2 h with geldanamycin (lower panel) are shown. Scale bars: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27029001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005962-m01_mouse-monoclonal-radixin-antibody-1e12-31020241548368.jpg "Western Blot: Radixin Antibody (1E12) [H00005962-M01] - ERM expression & localization in SKBR3 breast cancer cells(A) Western blot analysis of ERM levels in SKBR3, HeLa & PC3 cells. SKBR3 breast cancer cells do not express moesin. (B) Colocalization of ezrin/radixin & ErbB2 in SKBR3 cells. 3D-SIM of fixed cells, stained for endogenous ERM & ErbB2, shows a high degree of colocalization between the ezrin/radixin & ErbB2 at the plasma membrane (left panel: max. projection; middle: single plane section; right: single channels of insert). Scale bars: 10 μm. (C) Analysis of protein association in SKBR3 cells by proximity ligation assay (PLA). 2 h treatment with 3 μM GA leads to decreased association of ezrin/ErbB2 & radixin/ErbB2. Data is represented as mean +/− SEM (***P < 0.001). (D) Corresponding single plan section of a representative PLA experiment. Fluorescence & DIC pictures of control cells (upper panel) & cell treated for 2 h with geldanamycin (lower panel) are shown. Scale bars: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27029001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: Radixin Antibody (1E12) [H00005962-M01] - ERM expression & localization in SKBR3 breast cancer cells(A) Western blot analysis of ERM levels in SKBR3, HeLa & PC3 cells. SKBR3 breast cancer cells do not express moesin. (B) Colocalization of ezrin/radixin & ErbB2 in SKBR3 cells. 3D-SIM of fixed cells, stained for endogenous ERM & ErbB2, shows a high degree of colocalization between the ezrin/radixin & ErbB2 at the plasma membrane (left panel: max. projection; middle: single plane section; right: single channels of insert). Scale bars: 10 μm. (C) Analysis of protein association in SKBR3 cells by proximity ligation assay (PLA). 2 h treatment with 3 μM GA leads to decreased association of ezrin/ErbB2 & radixin/ErbB2. Data is represented as mean +/− SEM (***P < 0.001). (D) Corresponding single plan section of a representative PLA experiment. Fluorescence & DIC pictures of control cells (upper panel) & cell treated for 2 h with geldanamycin (lower panel) are shown. Scale bars: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27029001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005962-m01_mouse-monoclonal-radixin-antibody-1e12-31020241548225.jpg "Immunocytochemistry/ Immunofluorescence: Radixin Antibody (1E12) [H00005962-M01] - ERM expression & localization in SKBR3 breast cancer cells(A) Western blot analysis of ERM levels in SKBR3, HeLa & PC3 cells. SKBR3 breast cancer cells do not express moesin. (B) Colocalization of ezrin/radixin & ErbB2 in SKBR3 cells. 3D-SIM of fixed cells, stained for endogenous ERM & ErbB2, shows a high degree of colocalization between the ezrin/radixin & ErbB2 at the plasma membrane (left panel: max. projection; middle: single plane section; right: single channels of insert). Scale bars: 10 μm. (C) Analysis of protein association in SKBR3 cells by proximity ligation assay (PLA). 2 h treatment with 3 μM GA leads to decreased association of ezrin/ErbB2 & radixin/ErbB2. Data is represented as mean +/− SEM (***P < 0.001). (D) Corresponding single plan section of a representative PLA experiment. Fluorescence & DIC pictures of control cells (upper panel) & cell treated for 2 h with geldanamycin (lower panel) are shown. Scale bars: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27029001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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