![Western Blot: Rad51 Antibody (RAD51/2701) - Azide and BSA Free [NBP2-79895] - Western Blot Analysis of HeLa, HepG2, K562, A431 and MCF-7 cell lysate using Rad51 Antibody (RAD51/2701).](http://images.novusbio.com/fullsize/Rad51-Antibody-RAD51-2701-Azide-and-BSA-Free-Western-Blot-NBP2-79895-img0002.jpg "Western Blot: Rad51 Antibody (RAD51/2701) - Azide and BSA Free [NBP2-79895] - Western Blot Analysis of HeLa, HepG2, K562, A431 and MCF-7 cell lysate using Rad51 Antibody (RAD51/2701).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, IHC, MA, CyTOF-ready |
| Clone | RAD51/2701 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79760). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | Recombinant fragment of human Rad51 protein (around aa 1-134) (exact sequence is proprietary) (Uniprot: Q06609) |
| Localization | Nuclear |
| Marker | Prognostic and Response to Chemotherapy Marker |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RAD51 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Rad51 Antibody (NBP2-79895)Find related products by research area.
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The recent relationship of BRCA1 and 53BP1 The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage. DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m... Read full blog post. |
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RAD51: The cell's 'Mr. Fix-it' RAD51 is a recombinase protein encoded by RAD51 gene in humans. Human RAD51 family members are highly similar to bacterial RecA and yeast Rad51, both biochemically and structurally. It is a 339-amino acid protein that plays an important role in homolo... Read full blog post. |
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NUP153 & 53BP1: A Novel DNA Repair Pathway Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De... Read full blog post. |
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Determining DMC1's role in Homologus Recombination The DMC1 gene encodes a 36.7 kDa nuclear protein involved in meiotic homologous recombination. This recombinase is functionally related to the yeast RAD51 and E. coli RecA genes. In contrast to RAD51, which functions in both mitotic and meiotic recomb... Read full blog post. |
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Rad51 Antibody Reveals a Canine Model for Human Breast Cancer Our antibody catalog includes an extensive range of Rad51 antibody reagents. Encoded by the RAD51 gene, the Rad51 protein plays a vital role in DNA repair, interacting with several other proteins, including BRCA1 and BRCA2, to effect homologous recomb... Read full blog post. |
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Industrial Chemicals, Tumour Suppressor Genes and the Need for More Research Human cancer research is the largest research area in our antibody database, with new oncogenes and cell lines being added all the time.Cancer triggers come from many sources, with a worrying amount of evidence to suggest that chemicals we’re in con... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RAD51 |
![Flow Cytometry: Rad51 Antibody (RAD51/2701) - Azide and BSA Free [NBP2-79895] - Flow Cytometric Analysis of K562 cells using Rad51 Antibody (RAD51/2701)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Rad51-Antibody-RAD51-2701-Azide-and-BSA-Free-Flow-Cytometry-NBP2-79895-img0003.jpg "Flow Cytometry: Rad51 Antibody (RAD51/2701) - Azide and BSA Free [NBP2-79895] - Flow Cytometric Analysis of K562 cells using Rad51 Antibody (RAD51/2701)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Protein Array: Rad51 Antibody (RAD51/2701) - Azide and BSA Free [NBP2-79895] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Rad51 Antibody (RAD51/2701) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Rad51-Antibody-RAD51-2701-Azide-and-BSA-Free-Protein-Array-NBP2-79895-img0001.jpg "Protein Array: Rad51 Antibody (RAD51/2701) - Azide and BSA Free [NBP2-79895] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Rad51 Antibody (RAD51/2701) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.")
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