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Rad50 Antibody (684945) [Unconjugated]

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cells, HepG2 human hepatocellular carcinoma cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Rad50 Monoclonal Antibody (Catalog # MAB4996) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Rad50 at approximately ~150kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cells, HepG2 human hepatocellular carcinoma cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Rad50 Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
684945
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4996-SP
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Rad50 Antibody (684945) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Rad50
Leu518-Leu881
Accession # Q92878
Specificity
Detects human Rad50 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Rad51 or rhRad53 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RAD50
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rad50 Antibody (684945) [Unconjugated]

  • DNA repair protein RAD50
  • EC 3.6
  • EC 3.6.1.15
  • EC 3.6.3
  • EC 3.6.3.27
  • hRad50
  • NBSLD
  • RAD50 (S. cerevisiae) homolog
  • RAD50 homolog (S. cerevisiae)
  • Rad50
  • RAD502
  • RAD50-2

Background

Rad50 is a 150 kDa member of the Rad50 subfamily, SMC (structural maintenance of chromosomes) family of DNA-associated genes.  It is ubiquitously expressed, and associates with MRE11 and NBS1 to form an MRN complex.  This complex stabilizes ATM kinase, thus contributing to DNA repair, and also participates in the suppression of DNA rereplication in dividing cells.  Human Rad50 is 1312 amino acids (aa) in length.  It has an apparent ATP binding site (aa 36-43) plus a coiled-coil region (aa 228-598) followed by a “zinc-hook” domain (aa 635-734) that mediates homodimerization.  There are multiple splice variants.  An alternate start site exists at Met140, there is a single Lys substitution for aa 723-1312, and three Lys substitute for aa 555-1312.  Over aa 518-881, human Rad50 is 96% aa identical to mouse Rad50.   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RAD50
Uniprot