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Rabphilin 3A [p Ser234] Antibody

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Western Blot: Rabphilin 3A [p Ser234] Antibody [NB300-743] - Western blot of brain lysate showing specific immunolabeling of the ~82k rabphilin 3A phosphorylated at Ser234.

Product Details

Summary
Reactivity Rt, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Rabphilin 3A [p Ser234] Antibody Summary

Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH. Accession # P47709
Modification
p Ser234
Specificity
Specific for endogenous levels of the ~82 kDa Rabphilin 3A protein phosphorylated at Ser234. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPH3A
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:400
  • Immunohistochemistry 1:400
  • Western Blot 1:1000
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Antigen Affinity-purified

Alternate Names for Rabphilin 3A [p Ser234] Antibody

  • Exophilin-1
  • KIAA0985exophilin-1
  • rabphilin 3A homolog (mouse)
  • rabphilin
  • rabphilin-3A

Background

Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994;Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington's disease(Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995;Foletti and Scheller, 2001).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RPH3A
Entrez
Uniprot