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RAB3IP Antibody (OTI5F2)

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Western Blot: RAB3IP Antibody (OTI5F2) [NBP2-45500] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB3IP .
Immunocytochemistry/ Immunofluorescence: RAB3IP Antibody (OTI5F2) [NBP2-45500] - RAB3IP Antibody (5F2) [NBP2-45500] - 3dpf zebrafish neuromast immunostained for Rab3IP (red) and counterstained with phalloidin (green). ...read more
Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RAB3IP mouse monoclonal antibody. (Heat-induced epitope ...read more
Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RAB3IP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM ...read more
Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RAB3IP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM ...read more
Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500] - Staining of paraffin-embedded Human colon tissue within the normal limits using anti-RAB3IP mouse monoclonal antibody. (Heat-induced epitope ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ZeSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI5F2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

RAB3IP Antibody (OTI5F2) Summary

Immunogen
Full length human recombinant protein of human RAB3IP (NP_071901) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RAB3IP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
51.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP2-45500 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RAB3IP Antibody (OTI5F2)

  • FLJ14660
  • FLJ22548
  • MGC71495
  • RAB3A interacting protein (rabin3)
  • rab-3A-interacting protein
  • Rab3A-interacting protein
  • RABIN3
  • rabin-3
  • SSX2 interacting protein
  • SSX2-interacting protein

Background

RAB3IP, also known as Rab-3A-interacting protein, is a 53 kDa 476 amino acid protein with 8 isoforms produced by alternative splicing. The primary function of RAB3IP is to change GDP proteins into GTP proteins. Current research on RAB3IP is being conducted in relation to vitelliform, macular dystrophy, pilocytic astrocytoma, anaplastic astrocytoma and astrocytoma. RAB3IP is linked to the vesicle docking, metaphase, exocytosis, mitosis, transport, synaptic transmission and cytokinesis pathways where it interacts with RAB3IL1, SSX2B, SSX2, RAB8A and RAB3A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RAB3IP Antibody (NBP2-45500) (0)

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Review for RAB3IP Antibody (NBP2-45500) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Zebrafish.

Reviews using NBP2-45500:
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IF
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence RAB3IP NBP2-45500
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3
reviewed by:
Verified Customer
IF Zebrafish 02/28/2017
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Summary

ApplicationImmunofluorescence
Sample Testedwhole mount zebrafish
SpeciesZebrafish

Comments

Comments3-5dpf larvae were fixed at 4⁰C overnight with 4% PFA in PBS and then rinsed twice with PBS + Triton X-100 0.1%. Primary antibody was diluted in PBSTx 0.1% containing 2.5% BSA and 2.5% FBS at 1:200 dilution and samples incubated overnight with rocking at 4⁰C. After several washes larvae were incubated with AlexaFluor 594-mouse conjugated antibody and AlexaFluor 488-phalloidin.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RAB3IP Antibody (NBP2-45500) (0)

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mFluor Violet Conjugated Antibodies

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Verified Customer
02/28/2017
Application: IF
Species: Zebrafish

Bioinformatics

Gene Symbol RAB3IP