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Rab25 Antibody (3F12F3) - BSA Free

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Western Blot: Rab25 Antibody (3F12F3) [NBP1-28845] - Western blot analysis using Rab25 mouse mAb against truncated Rab25 recombinant protein (1), human overy carcinoma(2), stomach carcinoma (3), breast carcinoma (4) ...read more
Immunohistochemistry: Rab25 Antibody (3F12F3) [NBP1-28845] - analysis of paraffin-embedded human bladder carcinoma(A), breast carcinoma(B), esophagus carcinoma(C), skin carcinoma(D) tissue, showing cytoplasmic ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
3F12F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Rab25 Antibody (3F12F3) - BSA Free Summary

Immunogen
Purified recombinant fragment of Rab25 expressed in E. Coli.
Specificity
Specific for Rab25.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
RAB25
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
ELISA, Western Blot, Immunohistochemistry-Paraffin and Immunohistochemistry-Frozen
Publications
Read Publication using NBP1-28845.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Rab25 Antibody (3F12F3) - BSA Free

  • CATX8
  • CATX-8RAB11C
  • RAB11C
  • Rab25
  • RAB25, member RAS oncogene family
  • ras-related protein Rab-25

Background

Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rab25 Antibody (NBP1-28845)(1)

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Bioinformatics

Gene Symbol RAB25