![Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Sample (50 ug of whole cell lysate) A: Mouse Heart, 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Western-Blot-NBP2-20024-img0003.jpg "Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Sample (50 ug of whole cell lysate) A: Mouse Heart, 10% SDS PAGE gel, diluted at 1:10000.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Pyruvate Dehydrogenase E1 beta subunit. The exact sequence is proprietary. |
| Localization | Mitochondrion matrix |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PDHB |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 39 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
![Western Blot Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free](https://images.novusbio.com/images/Pyruvate-Dehydrogenase-E1-alpha-subunit-[p-Ser293]-Antibody-Western-Blot-NB110-93479-img0003.jpg)
![Western Blot Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free](https://images.novusbio.com/images/Pyruvate-Dehydrogenase-E1-alpha-subunit-[p-Ser293]-Antibody-Western-Blot-NB110-93479-img0008.jpg)
![Western Blot Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free](https://images.novusbio.com/images/Pyruvate-Dehydrogenase-E1-alpha-subunit-[p-Ser293]-Antibody-Western-Blot-NB110-93479-img0007.jpg)
| Publication using NBP2-20024 | Applications | Species |
|---|---|---|
| Grimm M, Calgeer B, Teriete P et al. Targeting thiamine-dependent enzymes for metabolic therapies in oral squamous cell carcinoma? Clin Transl Oncol. 2015-07-16 [PMID: 26179749] (IHC-P, Human) | IHC-P | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Pyruvate Dehydrogenase E1 beta subunit Antibody (NBP2-20024)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Pyruvate Dehydrogenase E1 beta subunit protein stained by Pyruvate Dehydrogenase E1 beta subunit antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20024-img0006.jpg "Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Pyruvate Dehydrogenase E1 beta subunit protein stained by Pyruvate Dehydrogenase E1 beta subunit antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Pyruvate Dehydrogenase E1 beta subunit antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-20024-img0007.jpg "Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Pyruvate Dehydrogenase E1 beta subunit antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - A. 50 ug rat heart lysate/extract 10 % SDS-PAGE Pyruvate Dehydrogenase E1 beta subunit antibody dilution: 1:5000.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Western-Blot-NBP2-20024-img0001.jpg "Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - A. 50 ug rat heart lysate/extract 10 % SDS-PAGE Pyruvate Dehydrogenase E1 beta subunit antibody dilution: 1:5000.")
![Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Western-Blot-NBP2-20024-img0002.jpg "Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-20024-img0004.jpg "Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Pyruvate Dehydrogenase E1 beta subunit antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-20024-img0005.jpg "Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Pyruvate Dehydrogenase E1 beta subunit antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Pyruvate Dehydrogenase E1 beta subunit antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")