| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | HRP |
| Immunogen | Antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human PUMA isoform 1. The immunogen is located within the first 50 amino acids of PUMA. Amino Acid Squence: ARARQEGSSPEPVEG |
| Specificity | Human PUMA has 4 isoforms, including isoform 1 (193aa, 21kD), isoform 2 (131aa, 14kD), isoform 3 (101aa, 10kD) and isofrom 4 (261aa, 27kD). This antibody detects human isoform 1, and is predicted to detect mouse and rat PUMA (193aa, 21kD for both of them). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BBC3 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Peptide affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for PUMA Antibody (NBP1-76639H)Find related products by research area.
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Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis? Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death. Novus Biologicals offers a variet... Read full blog post. |
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Altered expression of BCL2 in cancer Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease. For example, too little cell death can promote cell overgrowth a... Read full blog post. |
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IRE1 alpha dependent apoptotic-signaling pathway Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg... Read full blog post. |
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Understanding Noxa Regulation of Apoptosis Noxa is a pro-apoptotic gene belonging to the Bcl2 protein family that is unique in that it contains only BH3 domain. The BH3-only subclass of proteins, including proteins like PUMA and Bim in addition to Noxa, regulate the remaining Bcl-2 family memb... Read full blog post. |
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| Gene Symbol | BBC3 |
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