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PTK9 Antibody (7C11) - BSA Free

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Western Blot: PTK9 Antibody (7C11) [NBP2-37456] - Western blot analysis using TWF1 mAb against HEK293 (1) and TWF1 (AA: 335-384)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: PTK9 Antibody (7C11) [NBP2-37456] - Immunofluorescence analysis of HeLa cells using TWF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: PTK9 Antibody (7C11) [NBP2-37456] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TWF1 mouse mAb with DAB staining.
Flow Cytometry: PTK9 Antibody (7C11) [NBP2-37456] - Flow cytometric analysis of HeLa cells using TWF1 mouse mAb (green) and negative control (red).
Western Blot: PTK9 Antibody (7C11) [NBP2-37456] - Western blot analysis using TWF1 mAb against human TWF1 recombinant protein. (Expected MW is 31.1 kDa)
Immunohistochemistry: PTK9 Antibody (7C11) [NBP2-37456] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TWF1 mouse mAb with DAB staining.
ELISA: PTK9 Antibody (7C11) [NBP2-37456] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
7C11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Catalog# & Formulation Size Price
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PTK9 Antibody (7C11) - BSA Free Summary

Immunogen
Purified recombinant fragment of human PTK9 (AA: 335-384) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TWF1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
40.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-37456 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for PTK9 Antibody (7C11) - BSA Free

  • A6 protein tyrosine kinase
  • A6
  • Protein A6
  • Protein tyrosine kinase 9MGC23788
  • PTK9 protein tyrosine kinase 9
  • PTK9MGC41876
  • twinfilin, actin-binding protein, homolog 1 (Drosophila)
  • twinfilin-1

Background

PTK9 encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PTK9 Antibody (NBP2-37456)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PTK9 Antibody (NBP2-37456) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TWF1