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PTK9 Antibody (1D12)

Images

 
Western Blot: PTK9 Antibody (1D12) [H00005756-M05] - Analysis of PTK9 expression in transfected 293T cell line by PTK9 monoclonal antibody (M05), clone 1D12. Lane 1: PTK9 transfected lysatE (43.9 KDa). Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: PTK9 Antibody (1D12) [H00005756-M05] - Analysis of monoclonal antibody to TWF1 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: PTK9 Antibody (1D12) [H00005756-M05] - Western blot analysis of PTK9 over-expressed 293 cell line, cotransfected with PTK9 Validated Chimera RNAi or non-transfected control. Blot probed with H00005756-M05. ...read more
ELISA: PTK9 Antibody (1D12) [H00005756-M05] - Detection limit for recombinant GST tagged PTK9 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, KD
Clone
1D12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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PTK9 Antibody (1D12) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
PTK9 (AAH22344, 80 a.a. ~ 252 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VAFPISREAFQALEKLNNRQLNYVQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYKHSHEGDYLESIVFIYSMPGYTCSIRERMLYSSCKSRLLEIVERQLQMDVIRKIEIDNGDELTADFLYEEVHPKQHAHKQSSAKPKGPAGKRGIRRLIRGPAETEATTD
Specificity
PTK9 - PTK9 protein tyrosine kinase 9
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TWF1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Western Blot 1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PTK9 Antibody (1D12)

  • A6 protein tyrosine kinase
  • A6
  • Protein A6
  • Protein tyrosine kinase 9MGC23788
  • PTK9 protein tyrosine kinase 9
  • PTK9MGC41876
  • twinfilin, actin-binding protein, homolog 1 (Drosophila)
  • twinfilin-1

Background

This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TWF1
Entrez
Uniprot