![Immunohistochemistry-Paraffin: PTH Antibody (PTH/1174) - Azide and BSA Free [NBP2-47729] - Human Parathyroid stained with PTH Monoclonal Antibody (PTH/1174).](http://images.novusbio.com/fullsize/PTH-Antibody-PTH-1174-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47729-img0001.jpg "Immunohistochemistry-Paraffin: PTH Antibody (PTH/1174) - Azide and BSA Free [NBP2-47729] - Human Parathyroid stained with PTH Monoclonal Antibody (PTH/1174).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | PTH/1174 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45114). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | A recombinant fragment around aa 32-115 of human mature-PTH-polypeptide (exact sequence is proprietary) (Uniprot: P01270) |
| Localization | Cytoplasmic and Secreted |
| Specificity | Epitope of this monoclonal antibody maps in the C-terminus of PTH, a hormone produced by the parathyroid gland that regulates the concentration of calcium and phosphorus in extracellular fluid. This hormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing their renal excretion. It is produced in the parathyroid gland as an 84 amino acid single chain polypeptide. It can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH); also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PTH |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for PTH Antibody (NBP2-47729)Find related products by research area.
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| Gene Symbol | PTH |
![Immunohistochemistry-Paraffin: PTH Antibody (PTH/1174) - Azide and BSA Free [NBP2-47729] - Human Parathyroid stained with PTH Monoclonal Antibody (PTH/1174).](http://images.novusbio.com/fullsize/PTH-Antibody-PTH-1174-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47729-img0002.jpg "Immunohistochemistry-Paraffin: PTH Antibody (PTH/1174) - Azide and BSA Free [NBP2-47729] - Human Parathyroid stained with PTH Monoclonal Antibody (PTH/1174).")
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