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PTH Antibody (3H9 + PTH/1175) [Janelia Fluor® 646] - C-terminus, N-terminal

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
3H9 + PTH/1175
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47731AF350
Alexa Fluor 405
NBP2-47731AF405
Alexa Fluor 488
NBP2-47731AF488
Alexa Fluor 532
NBP2-47731AF532
Alexa Fluor 594
NBP2-47731AF594
Alexa Fluor 647
NBP2-47731AF647
Alexa Fluor 700
NBP2-47731AF700
Alexa Fluor 750
NBP2-47731AF750
Biotin
NBP2-47731B
DyLight 350
NBP2-47731UV
DyLight 405
NBP2-47731V
DyLight 488
NBP2-47731G
DyLight 550
NBP2-47731R
DyLight 594
NBP2-47731DL594
DyLight 650
NBP2-47731C
DyLight 680
NBP2-47731FR
DyLight 755
NBP2-47731IR
FITC
NBP2-47731F
HRP
NBP2-47731H
Janelia Fluor 549
NBP2-47731JF549
Unconjugated
NBP2-45116-0.1mg
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45116-0.1mg
Azide and BSA Free
NBP2-47731-0.1mg
IHC-Prediluted
NBP2-48404
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PTH Antibody (3H9 + PTH/1175) [Janelia Fluor® 646] - C-terminus, N-terminal Summary

Immunogen
A synthetic peptide around aa 1-34 of human mature-PTH-polypeptide (exact sequence is proprietary) (3H9); A recombinant fragment around aa 32-115 of human mature PTH-polypeptide (exact sequence is proprietary) (Uniprot: P01270)
Localization
Cytoplasmic and Secreted
Specificity
Epitope of this monoclonal antibody maps in the C-terminus of PTH, a hormone produced by the parathyroid gland that regulates the concentration of calcium and phosphorus in extracellular fluid. This hormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing their renal excretion. It is produced in the parathyroid gland as an 84 amino acid single chain polypeptide. It can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH); also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
Isotype
IgG2b Kappa/IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PTH
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Predicted to react with Mouse. Rat. Rabbit. Bovine. Canine. Porcine. Deer. Orangutan.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for PTH Antibody (3H9 + PTH/1175) [Janelia Fluor® 646] - C-terminus, N-terminal

  • Parathormone
  • Parathyrin
  • parathyroid hormone 1
  • parathyroid hormone
  • PTH
  • PTH1

Background

Parathyroid hormone (PTH), which is also designated parathyrin, is an 84 amino acid single chain peptide that functions to regulate calcium metabolism by raising blood levels of calcium through various mechanisms. PTH stimulates bone formation to increase bone mass and strength in rats and humans. Within the PTH molecule, the essential activity is associated with the first 34 amino acids at the amino-terminus of the molecule. The gene encoding PTH maps to human chromosome 11p15.3-p15.1. Parathyroid hormone-related protein (PTH-rP) is an autocrine factor that is structurally related to PTH yet, unlike PTH, which is synthesized only by the parathyroid cells, PTH-rP is synthesized by several cell types. PTH-rP regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Isolated from the culture medium of a human lung cancer cell line, PTH-rP produces PTH-like effects that are characterized as humoral hypercalcemia of malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PTH