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PSMD11 Antibody

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Western Blot: PSMD11 Antibody [NBP1-46191] - Treatment of human skin fibroblasts with 6BIO activated proteostatic modules. Representative immunoblot analyses of the autophagy-related Beclin1 and the 26S proteasome ...read more
Independent Antibodies: Western Blot: PSMD11 Antibody [NBP1-46191] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. ...read more
Treatment of human skin fibroblasts with 6BIO activated proteostatic modules. (B) Representative immunoblot analyses of the autophagy-related Beclin1 and the 26S proteasome subunits Rpn6, Rpt6, alpha7 and beta5 ...read more

Product Details

Summary
Reactivity Hu, Mu, Dr, Rt, Bv, Xp, ZeSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

PSMD11 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 175 and 225 of human Proteasome (Prosome, Macropain) 26S Subunit, non-ATPase, 11 using the numbering given in entry NP_002806.2 (GeneID 5717).
Predicted Species
Rat (100%), Xenopus (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMD11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
Publications
Read Publications using
NBP1-46191 in the following applications:

  • B/N
    1 publication
  • WB
    7 publications

Reactivity Notes

X. tropicalis (100%). Use in Drosophila reported in scientific literature (PMID:31002009).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PSMD11 Antibody

  • MGC3844,26S proteasome regulatory subunit RPN6
  • p44.5,26S proteasome regulatory subunit 9
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 11,26S proteasome regulatory subunit p44.5
  • Rpn6,26S proteasome regulatory subunit S9
  • S9,26S proteasome non-ATPase regulatory subunit 11

Background

PSMD11 (Proteasome 26S non-ATPase subunit 11), also known as S9, Rpn6 or p44.5, has multi-subunit protease complexes consisting of 20S subunits composed of four seven-numbered rings with two outer rings containing alpha subunits and two central rings composed of beta subunits, and 19S caps of 6 ATPase and 11 non-ATPase subunits. PSMD11 is the main proteolytic enzyme that functions in ATP-dependent degradation of ubiquitinated proteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSMD11 Antibody (NBP1-46191)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Drosophila.

We have publications tested in 2 applications: B/N, WB.


Filter By Application
B/N
(1)
WB
(7)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
Drosophila
(3)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP1-46191 Applications Species
Gumeni S, Lamprou M, Evangelakou Z et al. Sustained Nrf2 Overexpression-Induced Metabolic Deregulation Can Be Attenuated by Modulating Insulin/Insulin-like Growth Factor Signaling Cells 2023-11-18 [PMID: 37998385]
Schlesser C, Meul T, Stathopoulos G, Meiners S. Metformin Induces Resistance of Cancer Cells to the Proteasome Inhibitor Bortezomib Biomolecules 2022-05-28 [PMID: 35740881] (B/N) B/N
Manola MS, Gumeni S, Trougakos IP Differential Dose- and Tissue-Dependent Effects of foxo on Aging, Metabolic and Proteostatic Pathways Cells 2021-12-18 [PMID: 34944088] (WB, Drosophila) WB Drosophila
Dina E, Sklirou A, Chatzigeorgiou S et al. An enriched polyphenolic extract obtained from the by-product of Rosa damascena hydrodistillation activates antioxidant and proteostatic modules Phytomedicine 2021-09-01 [PMID: 34619431] (WB, Human, Drosophila) WB Human, Drosophila
Nath SR, Yu Z, Gipson TA et al. Androgen receptor polyglutamine expansion drives age-dependent quality control defects and muscle dysfunction J. Clin. Invest. 2018-05-29 [PMID: 29809168] (WB, Mouse) WB Mouse
Sklirou AD, Gaboriaud-Kolar N, Papassideri I et al. 6-bromo-indirubin-3'-oxime (6BIO), a Glycogen synthase kinase-3B inhibitor, activates cytoprotective cellular modules and suppresses cellular senescence-mediated biomolecular damage in human fibroblasts Sci Rep 2017-09-15 [PMID: 28916781]
Tsakiri E, Gaboriaud-Kolar N, Iliaki K et al. The indirubin derivative 6-Bromoindirubin-3'-oxime (6BIO) activates proteostatic modules, reprograms cellular bioenergetics pathways and exerts anti-aging effects. Antioxid. Redox Signal. 2017-03-02 [PMID: 28253732]
Welk V, Coux O, Kleene V et al. Inhibition of Proteasome Activity Induces Formation of Alternative Proteasome Complexes. J. Biol. Chem. 2016-04-18 [PMID: 27129254] (WB, Human) WB Human
Berschneider K Connecting the functions of the proteasome and mitochondria in the lung Thesis 2016-01-01 (WB, Mouse) WB Mouse
Yazgili AS, Meul T, Welk V et al. In-gel proteasome assay to determine the activity, amount, and composition of proteasome complexes from mammalian cells or tissues STAR protocols 2021-06-18 [PMID: 34027484]
Show All 12 Publications.

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol PSMD11