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PSMD11 Antibody

Images

 
Western Blot: PSMD11 Antibody [NBP1-46191] - Treatment of human skin fibroblasts with 6BIO activated proteostatic modules. Representative immunoblot analyses of the autophagy-related Beclin1 and the 26S proteasome ...read more
Independent Antibodies: Western Blot: PSMD11 Antibody [NBP1-46191] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. ...read more

Product Details

Summary
Reactivity Hu, Mu, Dr, Rt, Bv, Xp, ZeSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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PSMD11 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 175 and 225 of human Proteasome (Prosome, Macropain) 26S Subunit, non-ATPase, 11 using the numbering given in entry NP_002806.2 (GeneID 5717).
Predicted Species
Rat (100%), Xenopus (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMD11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
Publications
Read Publications using
NBP1-46191 in the following applications:

Reactivity Notes

X. tropicalis (100%). Use in Drosophila reported in scientific literature (PMID:31002009).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PSMD11 Antibody

  • MGC3844,26S proteasome regulatory subunit RPN6
  • p44.5,26S proteasome regulatory subunit 9
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 11,26S proteasome regulatory subunit p44.5
  • Rpn6,26S proteasome regulatory subunit S9
  • S9,26S proteasome non-ATPase regulatory subunit 11

Background

PSMD11 (Proteasome 26S non-ATPase subunit 11), also known as S9, Rpn6 or p44.5, has multi-subunit protease complexes consisting of 20S subunits composed of four seven-numbered rings with two outer rings containing alpha subunits and two central rings composed of beta subunits, and 19S caps of 6 ATPase and 11 non-ATPase subunits. PSMD11 is the main proteolytic enzyme that functions in ATP-dependent degradation of ubiquitinated proteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSMD11 Antibody (NBP1-46191)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Drosophila.

We have publications tested in 2 applications: Block/Neutralize, WB.


Filter By Application
Block/Neutralize
(1)
WB
(7)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
Drosophila
(3)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP1-46191 Applications Species
Gumeni S, Lamprou M, Evangelakou Z et al. Sustained Nrf2 Overexpression-Induced Metabolic Deregulation Can Be Attenuated by Modulating Insulin/Insulin-like Growth Factor Signaling Cells 2023-11-18 [PMID: 37998385]
Schlesser C, Meul T, Stathopoulos G, Meiners S. Metformin Induces Resistance of Cancer Cells to the Proteasome Inhibitor Bortezomib Biomolecules 2022-05-28 [PMID: 35740881] (Block/Neutralize) Block/Neutralize
Manola MS, Gumeni S, Trougakos IP Differential Dose- and Tissue-Dependent Effects of foxo on Aging, Metabolic and Proteostatic Pathways Cells 2021-12-18 [PMID: 34944088] (WB, Drosophila) WB Drosophila
Dina E, Sklirou A, Chatzigeorgiou S et al. An enriched polyphenolic extract obtained from the by-product of Rosa damascena hydrodistillation activates antioxidant and proteostatic modules Phytomedicine 2021-09-01 [PMID: 34619431] (WB, Human, Drosophila) WB Human, Drosophila
Nath SR, Yu Z, Gipson TA et al. Androgen receptor polyglutamine expansion drives age-dependent quality control defects and muscle dysfunction J. Clin. Invest. 2018-05-29 [PMID: 29809168] (WB, Mouse) WB Mouse
Sklirou AD, Gaboriaud-Kolar N, Papassideri I et al. 6-bromo-indirubin-3'-oxime (6BIO), a Glycogen synthase kinase-3B inhibitor, activates cytoprotective cellular modules and suppresses cellular senescence-mediated biomolecular damage in human fibroblasts Sci Rep 2017-09-15 [PMID: 28916781]
Tsakiri E, Gaboriaud-Kolar N, Iliaki K et al. The indirubin derivative 6-Bromoindirubin-3'-oxime (6BIO) activates proteostatic modules, reprograms cellular bioenergetics pathways and exerts anti-aging effects. Antioxid. Redox Signal. 2017-03-02 [PMID: 28253732]
Welk V, Coux O, Kleene V et al. Inhibition of Proteasome Activity Induces Formation of Alternative Proteasome Complexes. J. Biol. Chem. 2016-04-18 [PMID: 27129254] (WB, Human) WB Human
Berschneider K Connecting the functions of the proteasome and mitochondria in the lung Thesis 2016-01-01 (WB, Mouse) WB Mouse
Yazgili AS, Meul T, Welk V et al. In-gel proteasome assay to determine the activity, amount, and composition of proteasome complexes from mammalian cells or tissues STAR protocols 2021-06-18 [PMID: 34027484]
Show All 12 Publications.

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol PSMD11