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PSMD6 Antibody

Images

 
Western Blot: PSMD6 Antibody [NBP1-81545] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: PSMD6 Antibody [NBP1-81545] - Staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PSMD6 Antibody [NBP1-81545] - Staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Western Blot: PSMD6 Antibody [NBP1-81545] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: PSMD6 Antibody [NBP1-81545] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.
Immunohistochemistry-Paraffin: PSMD6 Antibody [NBP1-81545] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: PSMD6 Antibody [NBP1-81545] - Staining of human skin shows weak to moderate nuclear positivity in squamous epithelial cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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PSMD6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NMAPYYEALCKSLDWQIDVDLLNKMKKANEDELKRLDEELEDAEKNLGESEIRDAMMAKAEYLCRIGDKEGALTAFRKTYDKTVALG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMD6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PSMD6 Recombinant Protein Antigen (NBP1-81545PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PSMD6 Antibody

  • 26S proteasome non-ATPase regulatory subunit 6
  • Breast cancer-associated protein SGA-113M
  • KIAA010726S proteasome regulatory subunit S10
  • p42A
  • p44S10
  • PFAAP4
  • Phosphonoformate immuno-associated protein 4
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 6,26S proteasome regulatory subunit RPN7
  • Proteasome regulatory particle subunit p44S10
  • Rpn7
  • S10
  • SGA-113M

Background

Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PSMD6