![Western Blot: PRPF31 Antibody (S01-7F1) [NBP3-19498] - Western blot detection of PRPF31 in C2C12, PC3 cell lysates using NBP3-19498 (1:1000 diluted). Predicted band size: 56kDa. Observed band size: 56kDa.](http://images.novusbio.com/fullsize/PRPF31-Antibody-S01-7F1-Western-Blot-NBP3-19498-img0002.jpg "Western Blot: PRPF31 Antibody (S01-7F1) [NBP3-19498] - Western blot detection of PRPF31 in C2C12, PC3 cell lysates using NBP3-19498 (1:1000 diluted). Predicted band size: 56kDa. Observed band size: 56kDa.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | S01-7F1 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.3 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide of human PRPF31 (Uniprot # Q8WWY3) |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | PRPF31 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 56 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA |
| Preservative | 0.01% Sodium Azide |
| Concentration | 0.3 mg/ml |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PRPF31 |
![Immunocytochemistry/Immunofluorescence: PRPF31 Antibody (S01-7F1) [NBP3-19498] - Immunofluorescence of PRPF31 (green) in Hela using NBP3-19498 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/PRPF31-Antibody-S01-7F1-Immunocytochemistry-Immunofluorescence-NBP3-19498-img0001.jpg "Immunocytochemistry/Immunofluorescence: PRPF31 Antibody (S01-7F1) [NBP3-19498] - Immunofluorescence of PRPF31 (green) in Hela using NBP3-19498 at dilution 1/50, and DAPI (blue)")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")