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NOP56 Antibody (CL2603)

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Genetic Strategies: Western Blot: NOP56 Antibody (CL2603) [NBP2-36778] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NOP56 antibody. Remaining ...read more
Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778] - Staining in MCF7 cell line with Anti-NOP56 monoclonal antibody, showing nucleoli-specific staining in green. Microtubule- and nuclear ...read more
Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human breast cancer shows strong nucleolar positivity in tumor cells.
Western Blot: NOP56 Antibody (CL2603) [NBP2-36778] - Lane 1: Marker [kDa]. Lane 2:Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778] - Staining in HeLa cell line showing nucleoli-specific staining in green. Microtubule-staining and nuclear probes are visualized in red and ...read more
Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human cell line A431 showing nucleoli-specific staining in green. Microtubule-staining and nuclear probes are visualized in red ...read more
Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778] - Staining in U2OS cell line with Anti-NOP56 monoclonal antibody, showing nucleoli-specific staining in green. Microtubule-staining and ...read more
Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778] - Staining in U251 cell line showing nucleoli-specific staining in green. Microtubule-staining and nuclear probes are visualized in red and ...read more
Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human prostate cancer shows strong nucleolar positivity in glandular cells.
Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human fallopian tube shows strong nucleolar immunoreactivity in glandular and connective tissue cell
Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human kidney shows strong nucleolar positivity in renal tubules.
Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human cervix shows strong nucleolar immunoreactivity in the epithelial cells.
Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human liver shows strong nucleolar positivity in hepatocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clone
CL2603
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

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NOP56 Antibody (CL2603) Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:EDPSISFSKPKKKKSFSKEELMSSDLEETAGSTS
Epitope
FSKEELMSSDLEETA
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NOP56
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NOP56 Recombinant Protein Antigen (NBP2-36778PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for NOP56 Antibody (CL2603)

  • NOL5A
  • NOP56 ribonucleoprotein homolog (yeast)
  • nucleolar protein 56
  • nucleolar protein 5A (56kD with KKE/D repeat)
  • nucleolar protein 5A (56kDa with KKE/D repeat)
  • Nucleolar protein 5A

Background

Nop56p is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Nop56p is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. The protein encoded by this gene is similar in sequence to Nop56p and is also found in the nucleolus. Multiple transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of most of them have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NOP56
Uniprot