![Western Blot: Prolactin Antibody (OTI6B1) - Azide and BSA Free [NBP2-73609] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Prolactin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Prolactin.](http://images.novusbio.com/fullsize/Prolactin-Antibody-OTI6B1-Azide-and-BSA-Free-Western-Blot-NBP2-73609-img0001.jpg "Western Blot: Prolactin Antibody (OTI6B1) - Azide and BSA Free [NBP2-73609] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Prolactin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Prolactin.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI6B1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human PRL (NP_000939) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PRL |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 25.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Prolactin Antibody (NBP2-73609)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PRL |
![Immunocytochemistry/Immunofluorescence: Prolactin Antibody (OTI6B1) - Azide and BSA Free [NBP2-73609] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Prolactin.](http://images.novusbio.com/fullsize/Prolactin-Antibody-OTI6B1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73609-img0005.jpg "Immunocytochemistry/Immunofluorescence: Prolactin Antibody (OTI6B1) - Azide and BSA Free [NBP2-73609] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Prolactin.")
![Immunohistochemistry: Prolactin Antibody (OTI6B1) - Azide and BSA Free [NBP2-73609] - Staining of paraffin-embedded Human endometrium tissue using anti-Prolactin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Prolactin-Antibody-OTI6B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73609-img0007.jpg "Immunohistochemistry: Prolactin Antibody (OTI6B1) - Azide and BSA Free [NBP2-73609] - Staining of paraffin-embedded Human endometrium tissue using anti-Prolactin mouse monoclonal antibody.")
![Flow Cytometry: Prolactin Antibody (OTI6B1) - Azide and BSA Free [NBP2-73609] - Analysis of Jurkat cells, using anti-Prolactin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Prolactin-Antibody-OTI6B1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73609-img0003.jpg "Flow Cytometry: Prolactin Antibody (OTI6B1) - Azide and BSA Free [NBP2-73609] - Analysis of Jurkat cells, using anti-Prolactin antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Immunocytochemistry/Immunofluorescence: Prolactin Antibody (OTI6B1) - Azide and BSA Free [NBP2-73609] - Staining of HepG2 cells using anti-Prolactin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Prolactin-Antibody-OTI6B1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73609-img0004.jpg "Immunocytochemistry/Immunofluorescence: Prolactin Antibody (OTI6B1) - Azide and BSA Free [NBP2-73609] - Staining of HepG2 cells using anti-Prolactin mouse monoclonal antibody.")
![Immunohistochemistry: Prolactin Antibody (OTI6B1) - Azide and BSA Free [NBP2-73609] - Staining of paraffin-embedded Human breast tissue using anti-Prolactin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Prolactin-Antibody-OTI6B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73609-img0006.jpg "Immunohistochemistry: Prolactin Antibody (OTI6B1) - Azide and BSA Free [NBP2-73609] - Staining of paraffin-embedded Human breast tissue using anti-Prolactin mouse monoclonal antibody.")
![Immunohistochemistry: Prolactin Antibody (OTI6B1) - Azide and BSA Free [NBP2-73609] - Staining of paraffin-embedded Human Ovary tissue using anti-Prolactin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Prolactin-Antibody-OTI6B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73609-img0008.jpg "Immunohistochemistry: Prolactin Antibody (OTI6B1) - Azide and BSA Free [NBP2-73609] - Staining of paraffin-embedded Human Ovary tissue using anti-Prolactin mouse monoclonal antibody.")
![Flow Cytometry: Prolactin Antibody (OTI6B1) - Azide and BSA Free [NBP2-73609] - Analysis of Hela cells, using anti-Prolactin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Prolactin-Antibody-OTI6B1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73609-img0002.jpg "Flow Cytometry: Prolactin Antibody (OTI6B1) - Azide and BSA Free [NBP2-73609] - Analysis of Hela cells, using anti-Prolactin antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
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