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Prolactin Antibody (127813) [Unconjugated]

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Western blot shows lysate of human pituitary tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Prolactin Monoclonal Antibody (Catalog # MAB6821) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysate of human pituitary tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Prolactin Monoclonal Antibody (Catalog # MAB6821) followed by HRP-conjugated Anti-Mouse IgG Secondary ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
127813
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6821-SP
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Prolactin Antibody (127813) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Prolactin
Leu29-Thr151
Accession # P01236
Specificity
Recognizes both the full-length (amino acids 29 - 229) and a truncated form (amino acids 29 - 151) of recombinant human Prolactin in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRL
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB6821 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prolactin Antibody (127813) [Unconjugated]

  • PRL
  • Prolactin

Background

Prolactin (PRL) is a neuroendocrine pituitary hormone that is synthesized by anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, natural killer cells, and breast cancer cells. The prolactin receptor is a transmembrane type I glycoprotein that belongs to the hematopoietic cytokine receptor family.

Prolactin receptor (PRL R) contains an extracellular, transmembrane, and intracellular domain. Transcriptional regulation of the PRL R gene results in several different species-dependent isoforms of PRL R being produced.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Prolactin Antibody (MAB6821)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRL
Uniprot