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Progesterone R/NR3C3 Antibody (PR484) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Simple Western, IHC, CyTOF-ready
Clone
PR484
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

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Progesterone R/NR3C3 Antibody (PR484) [Janelia Fluor® 646] Summary

Immunogen
Recombinant full-length human Progesterone R/NR3C3 protein (PGR) (Uniprot: P06401 )
Localization
Nucleus
Marker
Marker of Progestin Dependence
Specificity
This monoclonal antibody is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone. It is excellent for immunohistochemical staining of formalin/paraffin tissues.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PGR
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Progesterone R/NR3C3 Antibody (PR484) [Janelia Fluor® 646]

  • NR3C3
  • NR3C3PRNuclear receptor subfamily 3 group C member 3
  • PGR
  • Progesterone R
  • progesterone receptor
  • ProgesteroneR

Background

The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for Progesterone R/NR3C3 Antibody (NBP2-33325JF646). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in getting antibodies for PR (progesterone receptor) that would work in flow cytometry. Could you recommend some choices?
    • We do not currently have any antibodies directed towards the progesterone receptor that have been validated in flow cytometry, but any of our antibodies that work in ICC should work just fine in flow as they are essentially the same application. Please see this link for our Progesterone Receptor antibodies validated in ICC.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PGR