![Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (PR484) - Azide and BSA Free [NBP2-33325] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with Progesterone R/NR3C3 Mouse Monoclonal Antibody (PR484).](http://images.novusbio.com/fullsize/Progesterone-R-NR3C3-Antibody-PR484-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33325-img0004.jpg "Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (PR484) - Azide and BSA Free [NBP2-33325] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with Progesterone R/NR3C3 Mouse Monoclonal Antibody (PR484).")
| Reactivity | HuSpecies Glossary |
| Applications | Simple Western, IHC, CyTOF-ready |
| Clone | PR484 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-33324). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant full-length human Progesterone R/NR3C3 protein (PGR) (Uniprot: P06401 ) |
| Localization | Nucleus |
| Marker | Marker of Progestin Dependence |
| Specificity | This monoclonal antibody is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone. It is excellent for immunohistochemical staining of formalin/paraffin tissues. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PGR |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Progesterone R/NR3C3 Antibody (NBP2-33325)Find related products by research area.
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| Gene Symbol | PGR |
![Simple Western: Progesterone R/NR3C3 Antibody (PR484) - Azide and BSA Free [NBP2-33325] - Simple Western lane view shows a specific band for Progesterone R/NR3C3 in 0.2 mg/ml of T47d (left) and MCF-7 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Progesterone-R-NR3C3-Antibody-PR484-Azide-and-BSA-Free-Simple-Western-NBP2-33325-img0001.jpg "Simple Western: Progesterone R/NR3C3 Antibody (PR484) - Azide and BSA Free [NBP2-33325] - Simple Western lane view shows a specific band for Progesterone R/NR3C3 in 0.2 mg/ml of T47d (left) and MCF-7 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Simple Western: Progesterone R/NR3C3 Antibody (PR484) - Azide and BSA Free [NBP2-33325] - Electropherogram image of the corresponding Simple Western lane. Progesterone R/NR3C3 antibody was used at 10 ug/ml dilution of T47d and MCF-7 lysates(s) respectively.](http://images.novusbio.com/fullsize/Progesterone-R-NR3C3-Antibody-PR484-Azide-and-BSA-Free-Simple-Western-NBP2-33325-img0002.jpg "Simple Western: Progesterone R/NR3C3 Antibody (PR484) - Azide and BSA Free [NBP2-33325] - Electropherogram image of the corresponding Simple Western lane. Progesterone R/NR3C3 antibody was used at 10 ug/ml dilution of T47d and MCF-7 lysates(s) respectively.")
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