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PRAME Antibody (CL5146) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
CL5146
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-61148AF350
Alexa Fluor 405
NBP2-61148AF405
Alexa Fluor 488
NBP2-61148AF488
Alexa Fluor 532
NBP2-61148AF532
Alexa Fluor 594
NBP2-61148AF594
Alexa Fluor 647
NBP2-61148AF647
Alexa Fluor 700
NBP2-61148AF700
Alexa Fluor 750
NBP2-61148AF750
Allophycocyanin
NBP2-61148APC
Biotin
NBP2-61148B
CoraFluor 1
NBP2-61148CL1
DyLight 350
NBP2-61148UV
DyLight 405
NBP2-61148V
DyLight 488
NBP2-61148G
DyLight 550
NBP2-61148R
DyLight 594
NBP2-61148DL594
DyLight 650
NBP2-61148C
DyLight 680
NBP2-61148FR
DyLight 755
NBP2-61148IR
FITC
NBP2-61148F
HRP
NBP2-61148H
Janelia Fluor 525
NBP2-61148JF525
Janelia Fluor 549
NBP2-61148JF549
Janelia Fluor 646
NBP2-61148JF646
PE
NBP2-61148PE
PerCP
NBP2-61148PCP
Unconjugated
NBP2-61148
mFluor Violet 450 SE
NBP2-61148MFV450
mFluor Violet 500 SE
NBP2-61148MFV500
mFluor Violet 610 SE
NBP2-61148MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-61148
Azide and BSA Free
NBP3-44110
BSA Free
NBP2-61148
BSA Free
NBP2-61148-25ul
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PRAME Antibody (CL5146) [Janelia Fluor® 669] Summary

Immunogen
This antibody was developed using a recombinant protein derived from P78395, with the exact immunogen sequence remaining proprietary.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PRAME
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for PRAME Antibody (CL5146) [Janelia Fluor® 669]

  • cancer/testis antigen 130
  • MAPEOIP4CT130
  • melanoma antigen preferentially expressed in tumors
  • OIP-4
  • Opa-interacting protein 4
  • Opa-interacting protein OIP4
  • preferentially expressed antigen in melanoma
  • Preferentially expressed antigen of melanoma

Background

PRAME encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Recent data from Epping et.al., (Cell, Vol. 122: 835) suggest that overexpression of PRAME frequently observed in human cancers confers growth or survival advantages by antagonizing RAR signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PRAME