PLGF Antibody (1038864) [Unconjugated] Summary
Immunogen |
E. coli-derived human PlGF Ala21-Arg149 Accession # P49763-2 |
Specificity |
Detects human PIGF in direct ELISAs. |
Source |
N/A |
Isotype |
IgG2b |
Clonality |
Monoclonal |
Host |
Mouse |
Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry 8-25 ug/mL
|
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Reconstitution Instructions |
Reconstitute at 0.5 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for PLGF Antibody (1038864) [Unconjugated]
Background
Placenta Growth Factor (PlGF) is a member of the PDGF/VEGF
family of growth factors that share a conserved pattern of eight cysteines (1, 2). Alternative splicing results in at least three human mature PlGF
forms containing 131 (PlGF-1), 152 (PlGF-2), and 203 (PlGF-3)
amino acids (aa) respectively (1, 2). Only PlGF-2 contains a highly
basic heparin-binding 21 aa insert at the C-terminus (1). Human PlGF-1
shares 56%, 55%, 74% and 95% aa identity with the comparable isoform of mouse,
rat, canine, and equine PlGF, respectively. PlGF is mainly found
as variably glycosylated, secreted, 55-60 kDa disulfide linked homodimers (3).
Mammalian cells expressing PlGF include villous trophoblasts, decidual
cells, erythroblasts, keratinocytes, and some endothelial cells (1, 4-6).
Circulating PlGF increases during pregnancy, reaching a peak in
mid-gestation; this increase is attenuated in preeclampsia (7). However,
deletion of PlGF in
the mouse does not affect development or reproduction.
Postnatally, mice lacking PlGF show impaired angiogenesis in response to ischemia (8). PlGF binds and signals through VEGF R1/Flt-1 but not VEGF R2/Flk-1/KDR, while VEGF binds both but signals only
through the angiogenic receptor, VEGF R2. PlGF and VEGF therefore
compete for binding to VEGF R1, allowing high PlGF to discourage
VEGF/VEGF R1 binding and promote VEGF/VEGF R2-mediated angiogenesis (1, 4, 8, 9).
However, PlGF (especially PlGF-1) and some forms of VEGF can form
dimers that decrease the angiogenic effect of VEGF on VEGF R2 (3, 4). PlGF-2,
but not PLGF-1, shows heparin-dependent binding of Neuropilin (Npn)-1 and Npn-2
(10, 11). PlGF induces monocyte activation, migration, and production of
inflammatory cytokines and VEGF. These activities facilitate wound, bone
fracture, and cardiac repair, but also contribute to inflammation in active
sickle cell disease and atherosclerosis (5, 6, 8, 12-15). PlGF can also inhibit TIMP3 expression in the spleen, leading to
immune triggering of hypertension (16).
- Hauser, S. and H.A. Weich (1993) Growth Factors 9:259.
- Maglione, D. et al. (1993) Oncogene 8:925.
- Eriksson, A. et al. (2002) Cancer Cell 1:99.
- Ribatti, D. (2008) Angiogenesis 11:215.
- Oura, H. et al. (2003) Blood 101:560.
- Roncal, C. et al. (2010) Cardiovasc. Res. 86:29.
- Levine, R.J. et al. (2004) N. Engl. J. Med. 350:672.
- Carmeliet, P. et al. (2001) Nat. Med. 7:575.
- Autiero, M. et al. (2003) Nat. Med. 9:936.
- Migdal, M. et al. (1998) J. Biol. Chem. 273:22272.
- Cheng, L. et al. (2004) J. Biol. Chem. 279:30654.
- Perelman, N. et al. (2003) Blood 102:1506.
- Cianfarani, F. et al. (2006) Am. J. Pathol. 169:1167.
- Maes, C. et al. (2006) J. Clin. Invest. 116:1230.
- Iwasaki, H. et al. (2011) PLoS One 6:e24872.
- Carnevale, D. et al. (2014) Immunity 41:737.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu
Applications: ELISA
Species: Hu
Applications: Block, CyTOF-ready, Flow, IHC, WB
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
Species: Hu, Rt
Applications: IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, IHC, Neut, WB
Species: Hu
Applications: BA
Species: Mu
Applications: IHC, IHC-Fr, IHC-P, WB
Species: Hu
Applications: BA
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ICC/IF
Publications for PLGF Antibody (MAB2643) (0)
There are no publications for PLGF Antibody (MAB2643).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for PLGF Antibody (MAB2643) (0)
There are no reviews for PLGF Antibody (MAB2643).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
FAQs for PLGF Antibody (MAB2643) (0)
Secondary Antibodies
| |
Isotype Controls
|
Additional PLGF Products
Blogs on PLGF