![Western Blot: Pleckstrin Antibody (OTI6H8) - Azide and BSA Free [NBP2-73464] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Pleckstrin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Pleckstrin.](http://images.novusbio.com/fullsize/Pleckstrin-Antibody-OTI6H8-Azide-and-BSA-Free-Western-Blot-NBP2-73464-img0001.jpg "Western Blot: Pleckstrin Antibody (OTI6H8) - Azide and BSA Free [NBP2-73464] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Pleckstrin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Pleckstrin.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, CyTOF-ready |
| Clone | OTI6H8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human PLEK (NP_002655) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PLEK |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 40.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Pleckstrin Antibody (NBP2-73464)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PLEK |
![Flow Cytometry: Pleckstrin Antibody (OTI6H8) - Azide and BSA Free [NBP2-73464] - HEK293T cells transfected with either pCMV6-ENTRY Pleckstrin.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-Pleckstrin mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Pleckstrin-Antibody-OTI6H8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73464-img0002.jpg "Flow Cytometry: Pleckstrin Antibody (OTI6H8) - Azide and BSA Free [NBP2-73464] - HEK293T cells transfected with either pCMV6-ENTRY Pleckstrin.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-Pleckstrin mouse monoclonal, and then analyzed by flow cytometry.")
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