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Pleckstrin Antibody (OTI6H8)

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Western Blot: Pleckstrin Antibody (6H8) [NBP2-02300] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Pleckstrin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: Pleckstrin Antibody (6H8) [NBP2-02300] Staining of paraffin-embedded prostate tissue using anti-Pleckstrin mouse monoclonal antibody.
Flow Cytometry: Pleckstrin Antibody (6H8) [NBP2-02300] - HEK293T cells transfected with either pCMV6-ENTRY Pleckstrin.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Pleckstrin mouse ...read more
Immunohistochemistry-Paraffin: Pleckstrin Antibody (6H8) [NBP2-02300] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Pleckstrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Pleckstrin Antibody (6H8) [NBP2-02300] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-Pleckstrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Pleckstrin Antibody (6H8) [NBP2-02300] - Staining of paraffin-embedded Kidney tissue using anti-Pleckstrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Pleckstrin Antibody (6H8) [NBP2-02300] - Staining of paraffin-embedded liver tissue using anti-Pleckstrin mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI6H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.82 mg/ml

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Pleckstrin Antibody (OTI6H8) Summary

Immunogen
Full length human recombinant protein of human PLEK (NP_002655) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PLEK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
Theoretical MW
40.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.82 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pleckstrin Antibody (OTI6H8)

  • FLJ27168
  • p47
  • P47Platelet 47 kDa protein
  • pleckstrin

Background

Pleckstrin is the major protein kinase C substrate of platelets however its exact function is not known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PLEK
Entrez