| Reactivity | MuSpecies Glossary |
| Applications | WB |
| Clone | 337801 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse Kallikrein/KLKB1 Gly20-Ala638 Accession # NP_032481 |
| Specificity | Detects mouse Plasma Kallikrein/KLKB1 in direct ELISAs and Western blots. In Western blots, no cross‑reactivity with recombinant human (rh) Kallikrein/KLKB1, rhKallikrein 1, 3, 4, 5, 7, 8, 10, 11, 12, 13, 14, 15, recombinant mouse HGFA, rhFactor VI, rhFactor X, rhFactor XI, rhThrombin, or rhuPA is observed. |
| Source | N/A |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | KLKB1 |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. |
Plasma kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma by binding to high molecular weight (HMW) kininogen or as a free zymogen. Once activated by its physiological activator, factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine. It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase. Thus, it plays an important role in blood pressure regulation, fibrinolysis, and neutrophil activation (1). Mouse plasma kallikrein precursor contains a signal peptide (aa 1‑19) and a pro form sequence (aa 20‑638). Upon activation, the pro form is converted to a heavy chain and a light chain, which is linked by disulfide bonds and the latter contains the catalytic domain (2).
Secondary Antibodies |
Isotype Controls |
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