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PLA2G1B Antibody

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Immunohistochemistry-Paraffin: PLA2G1B Antibody [NBP2-32569] - Staining in human pancreas and skeletal muscle tissues using anti-PLA2G1B antibody. Corresponding PLA2G1B RNA-seq data are presented for the same tissues.
Orthogonal Strategies: Immunohistochemistry-Paraffin: PLA2G1B Antibody [NBP2-32569] - Staining in human pancreas and skeletal muscle tissues using anti-PLA2G1B antibody. Corresponding PLA2G1B RNA-seq data are ...read more
Immunocytochemistry/ Immunofluorescence: PLA2G1B Antibody [NBP2-32569] - Staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: PLA2G1B Antibody [NBP2-32569] - Staining of human pancreas shows high expression.
Immunohistochemistry-Paraffin: PLA2G1B Antibody [NBP2-32569] - Staining of human skeletal muscle shows low expression as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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PLA2G1B Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ADSGISPRAVWQFRKMIKCVIPGSDPFLEYNNYGCYCGL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLA2G1B
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PLA2G1B Protein (NBP2-32569PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PLA2G1B Antibody

  • Group IB phospholipase A2
  • Phosphatidylcholine 2-acylhydrolase 1B
  • phospholipase A2
  • phospholipase A2, group IB (pancreas)
  • PLA2
  • PLA2A
  • PLA2AMGC119834
  • PLA2EC 3.1.1.4
  • PLA2G1B
  • PPLA2
  • PPLA2MGC119835

Background

Phospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]) .[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-32569
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Bioinformatics

Gene Symbol PLA2G1B