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PKC mu Antibody

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Western Blot: PKC mu Antibody [H00005587-B01P] - Analysis of PRKD1 expression in transfected 293T cell line by PRKD1 polyclonal antibody. Lane 1: PRKD1 transfected lysate(100.32 KDa). Lane 2: Non-transfected lysate.
Western Blot: PKC mu Antibody [H00005587-B01P] - Analysis of PRKD1 expression in transfected 293T cell line by PRKD1 polyclonal antibody. Lane 1: PRKD1 transfected lysate(100.32 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PKC mu Antibody [H00005587-B01P] - Analysis of purified antibody to PRKD1 on HeLa cell. (antibody concentration 10 ug/ml)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

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PKC mu Antibody Summary

Description
Quality control test: Antibody reactive against mammalian transfected lysate.
Immunogen
PRKD1 (AAI60015.1, 1 a.a. - 912 a.a.) full-length human protein. MSAPPVLRPPSPLLPVAAAAAAAAAALVPGSGPGPAPFLAPVAAPVGGISFHLQIGLSREPVLLLQDSSGDYSLAHVREMACSIVDQKFPECGFYGMYDKILLFRHDPTSENILQLVKAASDIQEGDLIEVVLSASATFEDFQIRPHALFVHSYRAPAFCDHCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRRRRLSNVSLTGVSTIRTSSAELSTSAPDEPLLQKSPSESFIGREKRSNSQSYIGRPIHLDKILMSKVKVPHTFVIHSYTRPTVCQYCKKLLKGLFRQGLQCKDCRFNCHKRCAPKVPNNCLGEVTINGDLLSPGAESDVVMEEGSDDNDSERNSGLMDDMEEAMVQDAEMAMAECQNDSGEMQDPDPDHEDANRTISPSTSNNIPLMRVVQSVKHTKRKSSTVMKEGWMVHYTSKDTLRKRHYWRLDSKCITLFQNDTGSRYYKEIPLSEILSLEPVKTSALIPNGANPHCFEITTANVVYYVGENVVNPSSPSPNNSVLTSGVGADVARMWEIAIQHALMPVIPKGSSVGTGTNLHRDISVSISVSNCQIQENVDISTVYQIFPDEVLGSGQFGIVYGGKHRKTGRDVAIKIIDKLRFPTKQESQLRNEVAILQNLHHPGVVNLECMFETPERVFVVMEKLHGDMLEMILSSEKGRLPEHITKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRNKGYNRSLDMWSVGVIIYVSLSGTFPFNEDEDIHDQIQNAAFMYPPNPWKEISHEAIDLINNLLQVKMRKRYSVDKTLSHPWLQDYQTWLDLRELECKIGERYITHESDDLRWEKYAGEQGLQYPTHLINPSASHSDTPETEETEMKALGERVSIL
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
PRKD1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
It has been used for WB and Functional.
Publications
Read Publication using H00005587-B01P.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PKC mu Antibody

  • EC 2.7.11
  • nPKC-D1
  • nPKC-mu
  • PKCM
  • PKC-MU
  • PKD1
  • PKDEC 2.7.11.13
  • PRKCMPKC-mu
  • PRKD1
  • Protein kinase C mu type
  • protein kinase C, mu
  • Protein kinase D
  • protein kinase D1
  • serine/threonine-protein kinase D1

Background

PKC mu is a novel member of the subgroup of atypical protein kinase Cs (PKC). Deduced protein sequence shows strong homology to conserved domains of members of the PKC subfamily. In vitro phorbol ester binding studies and kinase assays with lysates of cells overexpressing PKC mu showed phorbol ester-independent kinase activity, autophosphorylation, and, in normal rat kidney (NRK) cells, predominant phosphorylation of a 30-kDa protein at serine residues. Data suggest a role of PKC mu in signal transduction pathways related to growth control (1). PKC mu is a cytosolic protein, which upon binding to the trans-Golgi network (TGN) regulates the fission of transport carriers specifically destined to the cell surface. (2). Results demonstrate that betagamma subunits of the heterotrimeric G proteins directly activates PKD by interacting with its pleckstrin homology domain (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PKC mu Antibody (H00005587-B01P)(1)


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Bioinformatics

Gene Symbol PRKD1
Uniprot