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PKA C-gamma Antibody (2E4) - BSA Free

Images

 
Western Blot: PKA C-gamma Antibody (2E4) [NBP2-37487] - Analysis using PRKACG mAb against human PRKACG (AA: 164-351) recombinant protein. (Expected MW is 47.1 kDa)
Immunohistochemistry-Paraffin: PKA C-gamma Antibody (2E4) [NBP2-37487] - Analysis of rectum cancer tissues using PRKACG mouse mAb with DAB staining.
Flow Cytometry: PKA C-gamma Antibody (2E4) [NBP2-37487] - Analysis of MCF-7 cells using PRKACG mouse mAb (green) and negative control (red).
Immunohistochemistry-Paraffin: PKA C-gamma Antibody (2E4) [NBP2-37487] - Analysis of liver cancer tissues using PRKACG mouse mAb with DAB staining.
ELISA: PKA C-gamma Antibody (2E4) [NBP2-37487] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clone
2E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

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PKA C-gamma Antibody (2E4) - BSA Free Summary

Immunogen
Purified recombinant fragment of human PKA C-gamma expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRKACG
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Theoretical MW
40.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP2-37487 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for PKA C-gamma Antibody (2E4) - BSA Free

  • cAMP-dependent protein kinase catalytic subunit gamma
  • EC 2.7.11
  • EC 2.7.11.11
  • KAPG
  • PKA C-gamma
  • PKACg
  • PRKACG
  • protein kinase, cAMP-dependent, catalytic, gamma
  • serine(threonine) protein kinase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PKA C-gamma Antibody (NBP2-37487) (0)

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Review for PKA C-gamma Antibody (NBP2-37487) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-37487:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot PKA C-gamma NBP2-37487
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3
reviewed by:
Verified Customer
WB Mouse 11/04/2019
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Summary

ApplicationWestern Blot
Sample TestedAdult lung
SpeciesMouse
Lot130508

Comments

CommentsProtein content from mouse primary endothelial cells was quantified by a BCA assay. Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membranes. Membranes were probed with primary antibody PKA C-gamma diluted 1:1000 in 5% BSA 2, before incubation with anti mouse secondary horseradish peroxidase-conjugated antibody 1:5000. Blots were visualised with Immobilon Western Chemiluminescence HRP Substrate and imaged with Syngene chemiluminescence imaging system.

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Secondary Antibodies

 

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Verified Customer
11/04/2019
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PRKACG