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Pit1 Antibody (PIT1/7262) - Azide and BSA Free

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Formalin-fixed, paraffin-embedded human pituitary stained with POU1F1 / PIT-1 Mouse Monoclonal Antibody (PIT1/7262). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human pituitary stained with POU1F1 / PIT-1 Mouse Monoclonal Antibody (PIT1/7262). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
PIT1/7262
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-20970AF350
Alexa Fluor 405
NBP3-20970AF405
Alexa Fluor 488
NBP3-20970AF488
Alexa Fluor 532
NBP3-20970AF532
Alexa Fluor 594
NBP3-20970AF594
Alexa Fluor 647
NBP3-20970AF647
Alexa Fluor 700
NBP3-20970AF700
Alexa Fluor 750
NBP3-20970AF750
Allophycocyanin
NBP3-20970APC
Biotin
NBP3-20970B
CoraFluor 1
NBP3-20970CL1
DyLight 350
NBP3-20970UV
DyLight 405
NBP3-20970V
DyLight 488
NBP3-20970G
DyLight 550
NBP3-20970R
DyLight 594
NBP3-20970DL594
DyLight 650
NBP3-20970C
DyLight 680
NBP3-20970FR
DyLight 755
NBP3-20970IR
FITC
NBP3-20970F
HRP
NBP3-20970H
Janelia Fluor 525
NBP3-20970JF525
Janelia Fluor 549
NBP3-20970JF549
Janelia Fluor 585
NBP3-20970JF585
Janelia Fluor 635
NBP3-20970JF635
Janelia Fluor 646
NBP3-20970JF646
Janelia Fluor 669
NBP3-20970JF669
PE
NBP3-20970PE
PerCP
NBP3-20970PCP
Unconjugated
NBP3-20299-100ug
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-20299-100ug
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Pit1 Antibody (PIT1/7262) - Azide and BSA Free Summary

Description
Positive Controls: Human pituitary or tonsil.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Non-hazardous. No MSDS required.
Immunogen
Recombinant human full-length Pit1 protein
Localization
Nucleus.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
POU1F1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1:10-1:20
Application Notes
Immunohistochemistry (Formalin-fixed): 1:10-1:20 for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
10mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Pit1 Antibody (PIT1/7262) - Azide and BSA Free

  • CPHD1
  • GHF1
  • GHF-1pituitary-specific positive transcription factor 1
  • Growth hormone factor 1
  • Pit-1
  • PIT1pituitary-specific transcription factor 1
  • POU class 1 homeobox 1
  • POU domain class 1, transcription factor 1
  • POU domain, class 1, transcription factor 1
  • POU1F1a

Background

The Pit1 gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol POU1F1