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Pit1 Antibody (PIT1/7262)

Images

 
Formalin-fixed, paraffin-embedded human pituitary stained with POU1F1 / PIT-1 Mouse Monoclonal Antibody (PIT1/7262). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
PIT1/7262
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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Pit1 Antibody (PIT1/7262) Summary

Description
Positive Controls: Human pituitary or tonsil.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Non-hazardous. No MSDS required.
Immunogen
Recombinant human full-length Pit1 protein
Localization
Nucleus.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
POU1F1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1:10-1:20
Application Notes
Immunohistochemistry (Formalin-fixed): 1:10-1:20 for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Pit1 Antibody (PIT1/7262)

  • CPHD1
  • GHF1
  • GHF-1pituitary-specific positive transcription factor 1
  • Growth hormone factor 1
  • Pit-1
  • PIT1pituitary-specific transcription factor 1
  • POU class 1 homeobox 1
  • POU domain class 1, transcription factor 1
  • POU domain, class 1, transcription factor 1
  • POU1F1a

Background

The Pit1 gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pit1 Antibody (NBP3-20299) (0)

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Product General Protocols

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FAQs for Pit1 Antibody (NBP3-20299) (0)

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol POU1F1