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Pin1 Antibody (3G8) - BSA Free

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Western Blot: Pin1 Antibody (3G8) [NBP1-22956] - Cell lysates of 293T, HeLa and Jurkat (each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Pin1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: Pin1 Antibody (3G8) [NBP1-22956] - Cell lysates of 293T, HeLa and Jurkat (each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Pin1 (1:500). Proteins were ...read more
Flow Cytometry: Pin1 Antibody (3G8) [NBP1-22956] - Analysis of Pin1 in Jurkat cell line, staining at2-5ug for 1x106cells (red line). The secondary antibody usedgoat anti-mouse IgG Alexa fluor 488 conjugate. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow
Clone
3G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-22956AF350
Alexa Fluor 405
NBP1-22956AF405
Alexa Fluor 488
NBP1-22956AF488
Alexa Fluor 532
NBP1-22956AF532
Alexa Fluor 594
NBP1-22956AF594
Alexa Fluor 647
NBP1-22956AF647
Alexa Fluor 700
NBP1-22956AF700
Alexa Fluor 750
NBP1-22956AF750
Allophycocyanin/Cy7
NBP1-22956APCCY7
Biotin
NBP1-22956B
CoraFluor 1
NBP1-22956CL1
DyLight 350
NBP1-22956UV
DyLight 405
NBP1-22956V
DyLight 488
NBP1-22956G
DyLight 550
NBP1-22956R
DyLight 594
NBP1-22956DL594
DyLight 650
NBP1-22956C
DyLight 680
NBP1-22956FR
DyLight 755
NBP1-22956IR
FITC
NBP1-22956F
HRP
NBP1-22956H
Janelia Fluor 525
NBP1-22956JF525
Janelia Fluor 549
NBP1-22956JF549
Janelia Fluor 585
NBP1-22956JF585
Janelia Fluor 635
NBP1-22956JF635
Janelia Fluor 646
NBP1-22956JF646
Janelia Fluor 669
NBP1-22956JF669
PE/Atto594
NBP1-22956PEATT594
PE/Cy5.5
NBP1-22956PECY55
PE/Cy7
NBP1-22956PECY7
mFluor Violet 450 SE
NBP1-22956MFV450
mFluor Violet 500 SE
NBP1-22956MFV500
mFluor Violet 610 SE
NBP1-22956MFV610
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Pin1 Antibody (3G8) - BSA Free Summary

Immunogen
Recombinant human Pin1 (1-163 aa) purified from E. coli
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PIN1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Pin1 Antibody (3G8) - BSA Free

  • dod
  • EC 5.2.1.8
  • peptidylprolyl cis/trans isomerase, NIMA-interacting 1
  • peptidyl-prolyl cis/trans isomerase, NIMA-interacting
  • peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
  • Peptidyl-prolyl cis-trans isomerase Pin1
  • Pin1
  • PPIase Pin1
  • prolyl isomerase
  • protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1
  • protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1
  • Rotamase Pin1
  • UBL5

Background

Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation. Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA ,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53 , and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PIN1
Entrez
Uniprot