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PIK3C2A Antibody

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Immunohistochemistry: PIK3C2A Antibody [NBP3-37948] - Immunohistochemistry analysis of paraffin-embedded Mouse heart using PIK3C2A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with ...read more
Western Blot: PIK3C2A Antibody [NBP3-37948] - Western blot analysis of various lysates using PIK3C2A Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 ...read more
Immunohistochemistry: PIK3C2A Antibody [NBP3-37948] - Immunohistochemistry analysis of paraffin-embedded Human liver damage using PIK3C2A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed ...read more
Immunoprecipitation: PIK3C2A Antibody [NBP3-37948] - Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug PIK3C2A antibody. Western blot was performed from the immunoprecipitate using PIK3C2A ...read more
Immunohistochemistry: PIK3C2A Antibody [NBP3-37948] - Immunohistochemistry analysis of paraffin-embedded Rat heart using PIK3C2A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with ...read more
Immunocytochemistry/ Immunofluorescence: PIK3C2A Antibody [NBP3-37948] - Immunofluorescence analysis of C6 cells using PIK3C2A Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PIK3C2A Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PIK3C2A (NP_002636.2).

Sequence:
MAQISSNSGFKECPSSHPEPTRAKDVDKEEALQMEAEALAKLQKDRQVTDNQRGFELSSSTRKKAQVYNKQDYDLMVFPESDSQKRALDIDVEKLTQAELEKLLLDDSFETKKTPVLPVTPILSPSFSAQLYFRPTIQRGQWPPGLPGPSTYALPSIYPSTYSKQAAFQNGFNPRMPTFPSTEPIYLSLPGQSPYFSYPL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIK3C2A
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Immunoprecipitation 0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells
  • Western Blot 1:500 - 1:2000
Theoretical MW
191 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for PIK3C2A Antibody

  • C2-containing phosphatidylinositol kinase
  • CPK
  • DKFZp686L193
  • EC 2.7.1
  • EC 2.7.1.137
  • EC 2.7.1.153
  • EC 2.7.1.154
  • MGC142218
  • phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha
  • Phosphoinositide 3-kinase-C2-alpha
  • phosphoinositide-3-kinase, class 2, alpha polypeptide
  • PI3-K-C2(ALPHA)
  • PI3-K-C2A
  • PI3K-C2alpha
  • PI3K-C2-alpha
  • PIK3C2A
  • PtdIns-3-kinase C2 subunit alpha

Background

PIK3C2A belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PIK3C2A