![Western Blot: PIEZO1 Antibody [NBP2-10504] - PC2, TRPV4, & PIEZO1 localize to MLO-Y4 primary cilia & plasma membrane. (A–C) Localization of PC2, TRPV4, & PIEZO1 on MLO-Y4 primary cilia & plasma membrane. Acetylated alpha-tubulin is in enriched primary cilia. Scale bars, 10 μm. (D) Chemiluminescence-detected Western blot of PC2, TRPV4, PIEZO1, & actin protein in MLO-Y4s treated with scrambled control siRNA & Pkd2, Trpv4, or Piezo1 siRNA. (E) Quantified knockdown of Pkd2 (n = 4), Trpv4 (n = 10), & Piezo1 (n = 4) mRNA expression in MLO-Y4 cells treated with siRNA relative to controls (n = 10). Error bars show ±SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26029358), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-10504_rabbit-polyclonal-piezo1-antibody-31020241537199.jpg "Western Blot: PIEZO1 Antibody [NBP2-10504] - PC2, TRPV4, & PIEZO1 localize to MLO-Y4 primary cilia & plasma membrane. (A–C) Localization of PC2, TRPV4, & PIEZO1 on MLO-Y4 primary cilia & plasma membrane. Acetylated alpha-tubulin is in enriched primary cilia. Scale bars, 10 μm. (D) Chemiluminescence-detected Western blot of PC2, TRPV4, PIEZO1, & actin protein in MLO-Y4s treated with scrambled control siRNA & Pkd2, Trpv4, or Piezo1 siRNA. (E) Quantified knockdown of Pkd2 (n = 4), Trpv4 (n = 10), & Piezo1 (n = 4) mRNA expression in MLO-Y4 cells treated with siRNA relative to controls (n = 10). Error bars show ±SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26029358), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
| Reactivity | Hu, Mu, Rt, Po, RbSpecies Glossary |
| Applications | WB, ELISA, IHC, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | Synthetic peptide derived from C-terminal domain of human Protein FAM38A. |
| Specificity | Reacts with human 287 kDa Protein FAM38A. Cross-reacts with mouse, rat, rabbit and other species due to sequence homology. Piezo-type mechanosensitive ion channel component 1. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PIEZO1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris , 0.1M Glycine and 2% Sucrose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Protein A purified |
| Reconstitution Instructions | Lyophilized powder, reconstitute to 1 mg/ml in distilled water. |
Secondary Antibodies |
Isotype Controls |
Research Areas for PIEZO1 Antibody (NBP2-10504)Find related products by research area.
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Touch Infographic: From Touch Receptors to the Brain The body contains thousands of receptors and nerves which allow us to experience the sense of touch, also referred to as tactile perception. The somatosensory system allows organisms to perceive and decode a wide range of tactile stimuli to allow for ... Read full blog post. |
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PIEZO1: A Mechanosensitive Ion Channel Protein PIEZO1, and its close homologue PIEZO2, are mechanosensitive ion channel proteins. Mechanosensitive ion channels couple protein conformation to the mechanics of the surrounding membrane, and switch between a closed state and an open state in response ... Read full blog post. |
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