![Western Blot: Pescadillo Antibody [NB110-40548] - Detection of human Pescadillo by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pescadillo antibody NB110-40548 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Pescadillo-Antibody-Western-Blot-NB110-40548-img0005.jpg "Western Blot: Pescadillo Antibody [NB110-40548] - Detection of human Pescadillo by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pescadillo antibody NB110-40548 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 535 and the C-terminus (residue 588) of human Pescadillo using the numbering given in entry NP_055118.1 (GeneID 23481). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PES1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB110-40548 | Applications | Species |
|---|---|---|
| Li D, Yao C, Ding Z et al. Pescadillo ribosomal biogenesis factor 1 reduction suppresses tumour growth and renders chemosensitivity of head and neck squamous cell carcinoma Cancer medicine 2022-10-11 [PMID: 36217758] (IHC-P) | IHC-P |
Secondary Antibodies |
Isotype Controls |
Research Areas for Pescadillo Antibody (NB110-40548)Find related products by research area.
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| Gene Symbol | PES1 |
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![Immunohistochemistry: Pescadillo Antibody [NB110-40548] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Pescadillo used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Pescadillo-Antibody-Immunohistochemistry-NB110-40548-img0004.jpg "Immunohistochemistry: Pescadillo Antibody [NB110-40548] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Pescadillo used at a dilution of 1:200 (1ug/ml). Detection: DAB")
![Western Blot: Pescadillo Antibody [NB110-40548] - Detection of Human Pescadillo on HeLa whole cell lysate using NB110-40548. Pescadillo was also immunoprecipitated by another rabbit anti-Pescadillo antibody and NB110-40548.](http://images.novusbio.com/fullsize/Pescadillo-Antibody-Western-Blot-NB110-40548-img0002.jpg "Western Blot: Pescadillo Antibody [NB110-40548] - Detection of Human Pescadillo on HeLa whole cell lysate using NB110-40548. Pescadillo was also immunoprecipitated by another rabbit anti-Pescadillo antibody and NB110-40548.")
![Immunohistochemistry-Paraffin: Pescadillo Antibody [NB110-40548] - FFPE section of human breast adenocarcinoma. Affinity purified rabbit anti-Pescadillo used at a dilution of 1:250.](http://images.novusbio.com/fullsize/Pescadillo-Antibody-Immunohistochemistry-Paraffin-NB110-40548-img0001.jpg "Immunohistochemistry-Paraffin: Pescadillo Antibody [NB110-40548] - FFPE section of human breast adenocarcinoma. Affinity purified rabbit anti-Pescadillo used at a dilution of 1:250.")
![Immunoprecipitation: Pescadillo Antibody [NB110-40548] - Detection of human Pescadillo by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pescadillo antibody NB110-40548 used for IP at 3 ug per reaction. Pescadillo was also immunoprecipitated by another rabbit anti-Pescadillo antibody. For blotting immunoprecipitated Pescadillo, NB110-40548 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Pescadillo-Antibody-Immunoprecipitation-NB110-40548-img0006.jpg "Immunoprecipitation: Pescadillo Antibody [NB110-40548] - Detection of human Pescadillo by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pescadillo antibody NB110-40548 used for IP at 3 ug per reaction. Pescadillo was also immunoprecipitated by another rabbit anti-Pescadillo antibody. For blotting immunoprecipitated Pescadillo, NB110-40548 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")