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Peroxiredoxin 6 Antibody

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Orthogonal Strategies: Western Blot: Peroxiredoxin 6 Antibody [NBP1-90332] - Analysis in human cell lines PC-3 and MCF-7 using anti-PRDX6 antibody. Corresponding PRDX6 RNA-seq data are presented for the same cell ...read more
Immunohistochemistry-Paraffin: Peroxiredoxin 6 Antibody [NBP1-90332] - Staining of human stomach shows strong nuclear and cytoplasmic positivity in glandular cells.
Western Blot: Peroxiredoxin 6 Antibody [NBP1-90332] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Peroxiredoxin 6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSKDINAYNCEEPTEKLPFPIIDDRNRELAILLGMLDPAEKDEKGMPVTARVVFVFGPDKKLKLSILYPA
Predicted Species
Mouse (92%), Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRDX6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Peroxiredoxin 6 Protein (NBP1-90332PEP)
Publications
Read Publication using NBP1-90332.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 20647035)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Peroxiredoxin 6 Antibody

  • 1-Cys
  • 24 kDa protein
  • Acidic calcium-independent phospholipase A2
  • aiPLA2
  • aiPLA21-Cys PRX
  • Antioxidant protein 2
  • AOP2
  • AOP2Non-selenium glutathione peroxidase
  • EC 1.11.1.15
  • EC 1.11.1.7
  • EC 3.1.1.-
  • KIAA0106Red blood cells page spot 12
  • Liver 2D page spot 40
  • MGC46173
  • NSGPx
  • NSGPx1-Cys peroxiredoxin
  • p29
  • Peroxiredoxin 6
  • peroxiredoxin-6
  • PRDX6
  • PRX

Background

FUNCTION: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. CATALYTIC ACTIVITY: 2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH. SUBUNIT: Homotetramer. May interact with HTR2A. SUBCELLULAR LOCATION: Cytoplasm. Lysosome. Note=Also found in lung secretory organelles. MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin . MISCELLANEOUS: Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress. SIMILARITY: Belongs to the ahpC/TSA family. Rehydrin subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Peroxiredoxin 6 Antibody (NBP1-90332)(1)


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRDX6