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Rat PEMT ELISA Kit (Colorimetric)

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ELISA: Rat PEMT ELISA Kit (Colorimetric) [NBP2-81240] - Samples were spiked with high concentrations of Rat PEMT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat PEMT ELISA Kit (Colorimetric) [NBP2-81240] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL

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Rat PEMT ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat PEMT in samples. No significant cross-reactivity or interference between Rat PEMT and analogues was observed.
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.12%
Intra-Assay
CV% < 5.58%
Spike Recovery
86-103%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
PEMT

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat PEMT ELISA Kit (Colorimetric)

  • EC 2.1.1.17
  • PEMPTMGC2483
  • PEMT2PEAMT
  • phosphatidylethanolamine N-methyltransferase
  • PNMT

Background

PEMT also known as Phosphatidylethanolamine N-methyltransferase, has 3 isoforms, a 199 amino acid isoform that is 22 kDa, a 236 amino acid that is 26 kDa, and 232 amino acid isoform that is 25 kDa, converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver and localizes to the endoplasmic reticulum and mitochondria-associated membranes. This protein is being studied for its involvement in ceroid lipofuscinosis, neuronal ceroid-lipofuscinosis, smith-magenis syndrome, spina bifida, orofacial cleft, whiplash, fatty liver disease, liver disease, cystic fibrosis, hepatocellular carcinoma, Alzheimer's disease, gigantism, breast cancer, liver cancer, homocysteine, fibrosis, alcoholism, endometriosis, gastric cancer, and infertility. The protein has been linked to the glycerophospholipid biosynthesis, metabolism, phospholipid metabolism, synthesis of PC, metabolism of lipids and lipoproteins, acetylcholine synthesis, and acetylcholine synthesis pathways where it interacts with CALM1, CALM2, CALM3, ALG5, ALG6, ALG8, EMC3, FEN1, and over 180 other proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PEMT