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PDLIM3 Antibody

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Western Blot: PDLIM3 Antibody [NBP2-19755] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PDLIM3 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: PDLIM3 Antibody [NBP2-19755] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PDLIM3 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was ...read more
Western Blot: PDLIM3 Antibody [NBP2-19755] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PDLIM3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody ...read more
Western Blot: PDLIM3 Antibody [NBP2-19755] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HepG2 10% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Bv, RMSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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PDLIM3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PDLIM3. The exact sequence is proprietary.
Localization
Cytoplasm; myofibril; sarcomere; Z-disk
Predicted Species
Bovine (93%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDLIM3
Purity
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:20000
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (80%), Pig (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for PDLIM3 Antibody

  • Actinin-associated LIM protein
  • ALPFLJ26096
  • Alpha-actinin-2-associated LIM protein
  • DKFZp686L0362
  • enigma homolog
  • PDZ and LIM domain 3
  • PDZ and LIM domain protein 3

Background

The protein encoded by this gene contains a PDZ domain and a LIM domain, indicating that it may be involved in cytoskeletal assembly. In support of this, the encoded protein has been shown to bind the spectrin-like repeats of alpha-actinin-2 and to colocalize with alpha-actinin-2 at the Z lines of skeletal muscle. This gene is found near a region of chromosome 4 that has been implicated in facioscapulohumeral muscular dystrophy, but this gene does not appear to be involved in the disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol PDLIM3