Rat PDK4 ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Rat PDK4 in samples. No significant cross-reactivity or interference between Rat PDK4 and analogues was observed. |
Standard Curve Range |
0.16 - 10 ng/mL |
Sensitivity |
0.09 ng/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
CV% < 5.08% |
Intra-Assay |
CV% < 5.06% |
Spike Recovery |
86-103% |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
PDK4 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Rat PDK4 ELISA Kit (Colorimetric)
Background
Pyruvate dehydrogenase kinase isoform 4 (PDK4) is a component of the mitochondrial multienzyme complex (PHD) which is responsible for carbohydrate fuel regulation. PDK1 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
PDK4 antibodies are useful tools for glucose metabolism research and mitochondiral metabolism studies.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for PDK4 ELISA Kit (NBP2-81233). (Showing 1 - 1 of 1 FAQ).
-
Which PDK4 antibody do you recommend for immunoblot? I'm working with human cell lines.
- NBP1-07047 and NBP1-07049 are our best selling PDK4 antibodies that will meet your mentioned criteria i.e. Immunoblot/Western Blot in Human samples. However, all of our products are 100% guaranteed and you may find a complete list of PDK4 antibodies by searching PDK4 on our website and narrowing your results using the filters on the left-hand side of the screen.
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